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Literature summary extracted from

  • Cummings, J.A.; Nguyen, T.T.; Fedorov, A.A.; Kolb, P.; Xu, C.; Fedorov, E.V.; Shoichet, B.K.; Barondeau, D.P.; Almo, S.C.; Raushel, F.M.
    Structure, mechanism, and substrate profile for Sco3058: the closest bacterial homologue to human renal dipeptidase (2010), Biochemistry, 49, 611-622.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.13.19 expression in Escherichia coli BL21 Streptomyces coelicolor

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.13.19
-
 Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
3.4.13.19 D22H mutant with no residual enzyme acitvity Streptomyces coelicolor
3.4.13.19 D320A mutant with no residual enzyme acitvity Streptomyces coelicolor
3.4.13.19 D320N mutant with no residual enzyme acitvity Streptomyces coelicolor
3.4.13.19 H150A mutant with more than doubled Km-values Streptomyces coelicolor
3.4.13.19 H150N mutant with more than doubled Km-values Streptomyces coelicolor
3.4.13.19 R223K mutant with about 3fold reduced activity compared to wild-type enzyme Streptomyces coelicolor
3.4.13.19 R223M mutant with no residual enzyme acitvity Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.19 citrate
-
 Streptomyces coelicolor
3.4.13.19 phosphinate mimic of L-Ala-D-Asp
-
 Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.19 0.14
-
 L-Arg-D-Asp
-
 Streptomyces coelicolor
3.4.13.19 0.17
-
 L-Tyr-D-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 0.37
-
 L-Leu-D-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 0.55
-
 L-Leu-D-Glu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 0.56
-
 L-Met-D-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 0.75
-
 L-Leu-D-Ala
-
 Streptomyces coelicolor
3.4.13.19 0.89
-
 L-Met-D-Glu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 1.4
-
 L-Leu-L-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 1.8
-
 L-Arg-L-Asp wild-type enzyme, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 2
-
 L-Leu-L-Asp pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 2
-
 L-Leu-D-Ser pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 2.3
-
 L-Met-L-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 2.4
-
 L-Asn-D-Glu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 4.3
-
 L-Arg-L-Asp mutant H150A, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 4.4
-
 L-Ala-D-Ala pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 4.9
-
 L-Arg-L-Asp mutant H150N, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 8.8
-
 L-Arg-L-Asp mutant R223K, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 9.5
-
 L-Ala-L-Glu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 13.8
-
 L-Ala-L-Asp pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 16
-
 L-Ala-L-Gln pH 7.5, 30°C Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.19 Streptomyces coelicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.19 ammonium sulfate precipitation, size exclusion chromatography, ion exchange chromatography and gel filtration Streptomyces coelicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.19 D-Ala-D-Ala + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor D-Ala + D-Ala
-
 ?
3.4.13.19 D-Ala-L-Ala + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor D-Ala + L-Ala
-
 ?
3.4.13.19 Gly-D-Leu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor Gly + D-Leu
-
 ?
3.4.13.19 L-Ala-D-Ala + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + D-Ala
-
 ?
3.4.13.19 L-Ala-L-Ala + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + L-Ala
-
 ?
3.4.13.19 L-Ala-L-Arg + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + L-Arg
-
 ?
3.4.13.19 L-Ala-L-Asp + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + L-Asp
-
 ?
3.4.13.19 L-Ala-L-Gln + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + L-Gln
-
 ?
3.4.13.19 L-Ala-L-Glu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + L-Glu
-
 ?
3.4.13.19 L-Ala-L-His + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + L-His
-
 ?
3.4.13.19 L-Ala-L-Lys + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + L-Lys
-
 ?
3.4.13.19 L-Arg-D-Asp + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Arg + D-Asp
-
 ?
3.4.13.19 L-Arg-Gly + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Arg + Gly
-
 ?
3.4.13.19 L-Arg-L-Asp + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Arg + L-Asp
-
 ?
3.4.13.19 L-Asn-D-Glu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Asn + D-Glu
-
 ?
3.4.13.19 L-Leu-D-Ala + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Leu + D-Ala
-
 ?
3.4.13.19 L-Leu-D-Glu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Leu + D-Glu
-
 ?
3.4.13.19 L-Leu-D-Leu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Leu + D-Leu
-
 ?
3.4.13.19 L-Leu-D-Ser + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Leu + D-Ser
-
 ?
3.4.13.19 L-Leu-L-Asp + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Leu + L-Asp
-
 ?
3.4.13.19 L-Leu-L-Leu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Leu + L-Leu
-
 ?
3.4.13.19 L-Met-D-Glu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Met + D-Glu
-
 ?
3.4.13.19 L-Met-D-Leu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Met + D-Leu
-
 ?
3.4.13.19 L-Met-L-Leu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Met + L-Leu
-
 ?
3.4.13.19 L-Tyr-D-Leu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Tyr + D-Leu
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.13.19 renal dipeptidase
-
 Streptomyces coelicolor
3.4.13.19 Sco3058
-
 Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.13.19 30
-
 assay at Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.13.19 additional information
-
 L-Arg-L-Asp mutant D22H, value below 0.03, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 additional information
-
 L-Arg-L-Asp mutant D320A, value below 0.03, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 additional information
-
 L-Arg-L-Asp mutant D320N, value below 0.03, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 additional information
-
 L-Arg-L-Asp mutant R223M, value below 0.03, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 0.4
-
 L-Arg-L-Asp mutant R223K, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 0.7
-
 L-Arg-L-Asp mutant H150N, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 7.2
-
 L-Arg-L-Asp mutant H150A, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 21
-
 L-Leu-D-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 24
-
 L-Tyr-D-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 107
-
 L-Arg-D-Asp
-
 Streptomyces coelicolor
3.4.13.19 132
-
 L-Arg-L-Asp wild-type enzyme, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 135
-
 L-Leu-D-Glu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 140
-
 L-Leu-L-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 140
-
 L-Ala-L-Gln pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 160
-
 L-Leu-D-Ala
-
 Streptomyces coelicolor
3.4.13.19 160
-
 L-Leu-L-Asp pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 194
-
 L-Met-D-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 220
-
 L-Ala-L-Glu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 288
-
 L-Asn-D-Glu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 310
-
 L-Met-L-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 340
-
 L-Met-D-Glu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 400
-
 L-Leu-D-Ser pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 750
-
 L-Ala-D-Ala pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 1400
-
 L-Ala-L-Asp pH 7.5, 30°C Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.19 7.5
-
 assay at Streptomyces coelicolor

General Information

EC Number General Information Comment Organism
3.4.13.19 metabolism glutathione metabolism Streptomyces coelicolor

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.13.19 0.0022
-
 D-Ala-L-Ala pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 0.046
-
 L-Arg-L-Asp mutant R223K, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 0.072
-
 L-Ala-L-Lys pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 0.13
-
 D-Ala-D-Ala pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 0.14
-
 L-Arg-L-Asp mutant H150N, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 0.18
-
 L-Ala-L-Arg pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 0.45
-
 L-Ala-L-His pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 1.7
-
 L-Arg-L-Asp mutant H150A, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 8.4
-
 L-Ala-L-Gln pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 14
-
 L-Ala-L-Ala pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 22
-
 L-Arg-Gly pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 24
-
 L-Ala-L-Glu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 33
-
 Gly-D-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 56
-
 L-Leu-D-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 72
-
 L-Arg-L-Asp wild-type enzyme, pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 83
-
 L-Leu-L-Asp pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 100
-
 L-Leu-L-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 100
-
 L-Ala-L-Asp pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 120
-
 L-Asn-D-Glu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 140
-
 L-Met-L-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 150
-
 L-Tyr-D-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 170
-
 L-Ala-D-Ala pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 200
-
 L-Leu-D-Ser pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 210
-
 L-Leu-D-Ala pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 250
-
 L-Leu-D-Glu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 350
-
 L-Met-D-Leu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 380
-
 L-Met-D-Glu pH 7.5, 30°C Streptomyces coelicolor
3.4.13.19 760
-
 L-Arg-D-Asp pH 7.5, 30°C Streptomyces coelicolor