Literature summary extracted from
Liu, F.; Lee, H.J.; Strynadka, N.C.; Tanner, M.E.
Inhibition of Neisseria meningitidis sialic acid synthase by a tetrahedral intermediate analogue (2009), Biochemistry, 48, 9194-9201.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.5.1.56 |
expression in Escherichia coli (BL21 deltaDE3) with pCWori+ vector |
Neisseria meningitidis |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.5.1.56 |
purified and concentrated enzyme crystallized at 18°C, hanging-drop vapor diffusion, 10 mM MnCl2, 1.50-1.55 M malic acid, pH 6.2, soaked in 2 M sodium phosphate, pH 6.2, with 10 mM MnCl2, 25% ethylene glycol, frozen in liquid nitrogen at -173°C, complex with Mn2+ and 4.5/1 mixture of 2S-inhibitor/2R-inhibitor |
Neisseria meningitidis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.5.1.56 |
5-acetamido-4,6,7,8,9-pentahydroxy-2-phosphorylnonanoic acid ditriethylammonium salt |
4.5/1 mixture of 2S-inhibitor/2R-inhibitor, competitive inhibitor against phosphoenolpyruvate |
Neisseria meningitidis |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.5.1.56 |
0.157 |
- |
phosphoenolpyruvate |
37°C, 100 mM Tris-HCl buffer, pH 7.0, 1 mM MnCl2, 50 microM to 1 mM phosphoenolpyruvate, His-tagged enzyme, purine nucleoside phosphorylase, 200 mircoM 2-amino-6-mercapto-7-methylpurine ribonucleoside |
Neisseria meningitidis |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.5.1.56 |
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O |
Neisseria meningitidis |
- |
N-acetylneuraminate + phosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.5.1.56 |
Neisseria meningitidis |
E0NCD4 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.5.1.56 |
cell harvesting by centrifugation, resuspension, lysing in high-pressure homogenizer, centrifugation, chromatographic procedures including ion-exchange and gel filtration steps, concentration |
Neisseria meningitidis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.5.1.56 |
phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O |
- |
Neisseria meningitidis |
N-acetylneuraminate + phosphate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.5.1.56 |
NeuB |
- |
Neisseria meningitidis |
2.5.1.56 |
sialic acid synthase |
- |
Neisseria meningitidis |
Ki Value [mM]
EC Number |
Ki Value [mM] |
Ki Value maximum [mM] |
Inhibitor |
Comment |
Organism |
Structure |
---|
2.5.1.56 |
0.0031 |
- |
5-acetamido-4,6,7,8,9-pentahydroxy-2-phosphorylnonanoic acid ditriethylammonium salt |
4.5/1 mixture of 2S-inhibitor/2R-inhibitor, a 1.2/1 mixture of 2S-inhibitor/2R-inhibitor requires a 2.5-fold lower total inhibitor concentration, 37°C, 100 mM Tris-HCl buffer, pH 7.0, 1 mM MnCl2, 50 microM to 1 mM phosphoenolpyruvate, His-tagged enzyme, purine nucleoside phosphorylase, 200 mircoM 2-amino-6-mercapto-7-methylpurine ribonucleoside |
Neisseria meningitidis |
|
General Information
EC Number |
General Information |
Comment |
Organism |
---|
2.5.1.56 |
metabolism |
formation of sialic acid or N-acetylneuraminic acid to produce a capsular polysacccharide of polysialic acid to evade the host's immune system by mimicking its own cell surface polysaccharides |
Neisseria meningitidis |