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Literature summary extracted from

  • Liu, F.; Lee, H.J.; Strynadka, N.C.; Tanner, M.E.
    Inhibition of Neisseria meningitidis sialic acid synthase by a tetrahedral intermediate analogue (2009), Biochemistry, 48, 9194-9201.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.56 expression in Escherichia coli (BL21 deltaDE3) with pCWori+ vector Neisseria meningitidis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.56 purified and concentrated enzyme crystallized at 18°C, hanging-drop vapor diffusion, 10 mM MnCl2, 1.50-1.55 M malic acid, pH 6.2, soaked in 2 M sodium phosphate, pH 6.2, with 10 mM MnCl2, 25% ethylene glycol, frozen in liquid nitrogen at -173°C, complex with Mn2+ and 4.5/1 mixture of 2S-inhibitor/2R-inhibitor Neisseria meningitidis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.56 5-acetamido-4,6,7,8,9-pentahydroxy-2-phosphorylnonanoic acid ditriethylammonium salt 4.5/1 mixture of 2S-inhibitor/2R-inhibitor, competitive inhibitor against phosphoenolpyruvate Neisseria meningitidis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.56 0.157
-
phosphoenolpyruvate 37°C, 100 mM Tris-HCl buffer, pH 7.0, 1 mM MnCl2, 50 microM to 1 mM phosphoenolpyruvate, His-tagged enzyme, purine nucleoside phosphorylase, 200 mircoM 2-amino-6-mercapto-7-methylpurine ribonucleoside Neisseria meningitidis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.56 phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O Neisseria meningitidis
-
N-acetylneuraminate + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.56 Neisseria meningitidis E0NCD4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.56 cell harvesting by centrifugation, resuspension, lysing in high-pressure homogenizer, centrifugation, chromatographic procedures including ion-exchange and gel filtration steps, concentration Neisseria meningitidis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.56 phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O
-
Neisseria meningitidis N-acetylneuraminate + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.56 NeuB
-
Neisseria meningitidis
2.5.1.56 sialic acid synthase
-
Neisseria meningitidis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.56 0.0031
-
5-acetamido-4,6,7,8,9-pentahydroxy-2-phosphorylnonanoic acid ditriethylammonium salt 4.5/1 mixture of 2S-inhibitor/2R-inhibitor, a 1.2/1 mixture of 2S-inhibitor/2R-inhibitor requires a 2.5-fold lower total inhibitor concentration, 37°C, 100 mM Tris-HCl buffer, pH 7.0, 1 mM MnCl2, 50 microM to 1 mM phosphoenolpyruvate, His-tagged enzyme, purine nucleoside phosphorylase, 200 mircoM 2-amino-6-mercapto-7-methylpurine ribonucleoside Neisseria meningitidis

General Information

EC Number General Information Comment Organism
2.5.1.56 metabolism formation of sialic acid or N-acetylneuraminic acid to produce a capsular polysacccharide of polysialic acid to evade the host's immune system by mimicking its own cell surface polysaccharides Neisseria meningitidis