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Literature summary extracted from

  • Ariyananda, L.d.e..Z.; Lee, P.; Antonopoulos, C.; Colman, R.F.
    Biochemical and biophysical analysis of five disease-associated human adenylosuccinate lyase mutants (2009), Biochemistry, 48, 5291-5302.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.2 expressed in Escherichia coli Rosetta 2 (DE3) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.3.2.2 K246E the mutation is associated with adenylosuccinate lyase deficiency, the Vmax for the K246E mutant enzyme is about 2% that of the wild type enzyme, K246E exists mainly as dimer or monomer Homo sapiens
4.3.2.2 L311V the mutation is associated with adenylosuccinate lyase deficiency, the Vmax for the L311V mutant enzyme is 72% about 2% that of the wild type enzyme Homo sapiens
4.3.2.2 R194C the mutation is associated with adenylosuccinate lyase deficiency,Vmax (at 25°C) for R194C is comparable to that of wild type, R194C is rapidly inactivated at 60°C Homo sapiens
4.3.2.2 R396C the mutation is associated with adenylosuccinate lyase deficiency the Vmax for the R396C mutant enzyme is about 16% that of the wild type enzyme Homo sapiens
4.3.2.2 R396H the mutation is associated with adenylosuccinate lyase deficiency, Vmax (at 25°C) for R194C is considerably reduced and affinity for adenylosuccinate is retained Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.2 56000
-
 4 * 56000, analytical ultracentrifugation Homo sapiens
4.3.2.2 57000
-
 4 * 57000, SDS-PAGE Homo sapiens
4.3.2.2 223000
-
 gel filtration, at 22°C Homo sapiens
4.3.2.2 227000
-
 analytical ultracentrifugation, at 37°C Homo sapiens
4.3.2.2 230000
-
 analytical ultracentrifugation, at 25°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.2 5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide Homo sapiens
-
 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
4.3.2.2 succinyladenosine monophosphate Homo sapiens
-
 AMP + fumarate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.2 Homo sapiens P30566
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.2 Ni-NTA agarose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.2 5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide
-
 Homo sapiens 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
4.3.2.2 succinyladenosine monophosphate
-
 Homo sapiens AMP + fumarate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.2.2 homotetramer 4 * 57000, SDS-PAGE Homo sapiens
4.3.2.2 homotetramer 4 * 56000, analytical ultracentrifugation Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.3.2.2 ASL
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.2.2 60
-
 wild type and mutant enzymes R396H and R396C are relatively stable at 60°C for about 4 h, the R194C mutant enzyme loses most of its activity within about 1 h of incubation at 60 °C Homo sapiens