EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.2.99.21 | X-ray crystallographic structures for mutant K42A with salicylate and pyruvate bound, to 2.5 A resolution, and for apo-I87T, to 2.15 A resolution. Circular dichroism studies of mutants K42A, K42Q, K42E, and K42H, A43P and I87T | Pseudomonas aeruginosa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.99.21 | A43P | about 25% decrease in both chorismate mutase and isochorismate pyruvate lyase activity | Pseudomonas aeruginosa |
4.2.99.21 | I87T | structure demonstrates considerable mobility, decrease in both chorismate mutase and isochorismate pyruvate lyase activity | Pseudomonas aeruginosa |
4.2.99.21 | K42A | residue presumably involved in electrostatic transition state stabilization. Active site architecture is maintained in mutant K42A | Pseudomonas aeruginosa |
4.2.99.21 | K42E | almost complete loss of activity | Pseudomonas aeruginosa |
4.2.99.21 | K42H | strong decrease in activity | Pseudomonas aeruginosa |
4.2.99.21 | K42Q | almost complete loss of activity | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.99.21 | 0.0011 | - |
isochorismate | mutant I87T, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.0043 | - |
isochorismate | wild-type, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.0053 | - |
isochorismate | mutant A43P, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.051 | - |
isochorismate | mutant K42A, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.057 | - |
isochorismate | mutant K42H, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.066 | - |
isochorismate | mutant K42E, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.066 | - |
isochorismate | mutant K42H, pH 5.0, 25°C | Pseudomonas aeruginosa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.99.21 | Pseudomonas aeruginosa | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.99.21 | isochorismate | - |
Pseudomonas aeruginosa | salicylate + pyruvate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.99.21 | 0.0142 | - |
isochorismate | mutant I87T, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.0245 | - |
isochorismate | mutant K42A, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.037 | - |
isochorismate | mutant K42H, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.0468 | - |
isochorismate | mutant K42E, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.118 | - |
isochorismate | mutant K42H, pH 5.0, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.177 | - |
isochorismate | wild-type, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.188 | - |
isochorismate | mutant A43P, pH 7.5, 25°C | Pseudomonas aeruginosa |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.99.21 | 0.48 | - |
isochorismate | mutant K42A, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.65 | - |
isochorismate | mutant K42H, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 0.71 | - |
isochorismate | mutant K42E, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 13 | - |
isochorismate | mutant I87T, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 17.9 | - |
isochorismate | mutant K42H, pH 5.0, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 35.5 | - |
isochorismate | mutant A43P, pH 7.5, 25°C | Pseudomonas aeruginosa | |
4.2.99.21 | 41 | - |
isochorismate | wild-type, pH 7.5, 25°C | Pseudomonas aeruginosa |