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Literature summary extracted from

  • Nie, L.; Ren, Y.; Schulz, H.
    Identification and characterization of Escherichia coli thioesterase III that functions in fatty acid beta-oxidation (2008), Biochemistry, 47, 7744-7751.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.20 His-tagged version expressed in Escherichia coli BL21(DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.2.20
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.20 0.003
-
 3,5-cis-tetradecadienoyl-CoA
-
 Escherichia coli
3.1.2.20 0.0058
-
 3-hydroxytetradecanoyl-CoA
-
 Escherichia coli
3.1.2.20 0.006
-
 palmitoyl-CoA
-
 Escherichia coli
3.1.2.20 0.0063
-
 myristoyl-CoA
-
 Escherichia coli
3.1.2.20 0.0108
-
 3,5-cis-dodecadienoyl-CoA
-
 Escherichia coli
3.1.2.20 0.013
-
 lauroyl-CoA
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.20 15000
-
 4 * 15000, crystal structure analysis, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.20 Escherichia coli P77712
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.20 ion exchange chromatography (DEAE-cellulose), hydroxylapatite chromatography, hydrophobic interaction chromatography, recombinant protein: immobilized metal ion affinity chromatography (Ni2+), ion exchange chromatography (DEAE-cellulose), hydrophobic interaction chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.20 additional information
-
 also comparable activities observed with stearoyl-CoA and oleoyl-CoA Escherichia coli
3.1.2.20 6.9
-
 with lauroyl-CoA Escherichia coli
3.1.2.20 26.4
-
 with myristoyl-CoA Escherichia coli
3.1.2.20 43.5
-
 with palmitoyl-CoA Escherichia coli
3.1.2.20 45.5
-
 with 3-hydroxytetradecanoyl-CoA Escherichia coli
3.1.2.20 92.3
-
 with 3,5-cis-dodecadienoyl-CoA Escherichia coli
3.1.2.20 101.3
-
 with 3,5-cis-tetradecadienoyl-CoA Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.20 3,5-cis-dodecadienoyl-CoA + H2O
-
 Escherichia coli CoA + 3,5-cis-dodecadienoic acid
-
 ?
3.1.2.20 3,5-cis-tetradecadienoyl-CoA + H2O
-
 Escherichia coli CoA + 3,5-cis-tetradecadienoic acid
-
 ?
3.1.2.20 3-hydroxytetradecanoyl-CoA + H2O
-
 Escherichia coli CoA + 3-hydroxytetradecanoate
-
 ?
3.1.2.20 lauroyl-CoA + H2O
-
 Escherichia coli CoA + laurate
-
 ?
3.1.2.20 myristoyl-CoA + H2O
-
 Escherichia coli CoA + myristate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Escherichia coli CoA + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.20 homotetramer 4 * 15000, crystal structure analysis, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.2.20 thioesterase III
-
 Escherichia coli
3.1.2.20 ybaW protein
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.20 additional information
-
 additional information inactive with substrates having acyl chains with fewer than 12 carbon atoms Escherichia coli
3.1.2.20 1.6
-
 lauroyl-CoA
-
 Escherichia coli
3.1.2.20 2 3.7 3,5-cis-tetradecadienoyl-CoA
-
 Escherichia coli
3.1.2.20 6.2
-
 myristoyl-CoA
-
 Escherichia coli
3.1.2.20 10.2
-
 palmitoyl-CoA
-
 Escherichia coli
3.1.2.20 10.7
-
 3-hydroxytetradecanoyl-CoA
-
 Escherichia coli
3.1.2.20 21.6
-
 3,5-cis-dodecadienoyl-CoA
-
 Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.2.20 100
-
 lauroyl-CoA
-
 Escherichia coli
3.1.2.20 1000
-
 myristoyl-CoA
-
 Escherichia coli
3.1.2.20 1700
-
 palmitoyl-CoA
-
 Escherichia coli
3.1.2.20 1800
-
 3-hydroxytetradecanoyl-CoA
-
 Escherichia coli
3.1.2.20 2000
-
 3,5-cis-dodecadienoyl-CoA
-
 Escherichia coli
3.1.2.20 7900
-
 3,5-cis-tetradecadienoyl-CoA
-
 Escherichia coli