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Literature summary extracted from

  • Sinitsyna, O.A.; Fedorova, E.A.; Pravilnikov, A.G.; Rozhkova, A.M.; Skomarovsky, A.A.; Matys, V.Y.; Bubnova, T.M.; Okunev, O.N.; Vinetsky, Y.P.; Sinitsyn, A.P.
    Isolation and properties of xyloglucanases of Penicillium sp (2010), Biochemistry (Moscow), 75, 41-49.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.151 gene xegA, DNA and amino acid sequence determination and analysis Penicillium canescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.151 additional information
-
 additional information kinetics Talaromyces verruculosus
3.2.1.151 additional information
-
 additional information kinetics Penicillium canescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.151 extracellular the enzyme is secredted Penicillium canescens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.151 25000
-
 x * 25000, XG 25, SDS-PAGE, x * 70000, XG 70, SDS-PAGE, XG 25 and XG 70 are organized in an enzyme complex Talaromyces verruculosus
3.2.1.151 25000
-
 x * 25000, XG A, SDS-PAGE, x * 26154, XG A, sequence calculation Penicillium canescens
3.2.1.151 26154
-
 x * 25000, XG A, SDS-PAGE, x * 26154, XG A, sequence calculation Penicillium canescens
3.2.1.151 70000
-
 x * 25000, XG 25, SDS-PAGE, x * 70000, XG 70, SDS-PAGE, XG 25 and XG 70 are organized in an enzyme complex Talaromyces verruculosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.151 xyloglucan + H2O Talaromyces verruculosus xyloglucan is a widespread hemicellulose polysaccharide of plant cell walls. It contains mainly D-glucose and D-xylose in the ratio of approximately 4:3, less amount of D-galactose, and also can contain L-fucose and L-arabinose xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O Penicillium canescens xyloglucan is a widespread hemicellulose polysaccharide of plant cell walls. It contains mainly D-glucose and D-xylose in the ratio of approximately 4:3, less amount of D-galactose, and also can contain L-fucose and L-arabinose xyloglucan oligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.151 Penicillium canescens
-
 gene xegA encodes isozyme XG A
-
3.2.1.151 Talaromyces verruculosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.151 native extracellular enzyme from culture medium by anion exchange chromatography Penicillium canescens
3.2.1.151 native extracellular enzyme from culture medium by anion exchange chromatography to over 95% purity Talaromyces verruculosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.151 mycelium
-
 Penicillium canescens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.151 additional information
-
 comparison with specific activities of other xyloglucanases towards polysaccharide substrates, overview Talaromyces verruculosus
3.2.1.151 additional information
-
 comparison with specific activities of other xyloglucanases towards polysaccharide substrates, overview Penicillium canescens
3.2.1.151 3 8 purified XG 70, substrate is tamarind xyloglucan Talaromyces verruculosus
3.2.1.151 21
-
 purified XG A, substrate is tamarind xyloglucan Penicillium canescens
3.2.1.151 52
-
 purified XG 25, substrate is tamarind xyloglucan Talaromyces verruculosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.151 additional information the enzyme is organized in an enzyme complex Penicillium canescens ?
-
 ?
3.2.1.151 additional information XG 25 and XG 70 are organized in an enzyme complex Talaromyces verruculosus ?
-
 ?
3.2.1.151 xyloglucan + H2O xyloglucan is a widespread hemicellulose polysaccharide of plant cell walls. It contains mainly D-glucose and D-xylose in the ratio of approximately 4:3, less amount of D-galactose, and also can contain L-fucose and L-arabinose Talaromyces verruculosus xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O xyloglucan is a widespread hemicellulose polysaccharide of plant cell walls. It contains mainly D-glucose and D-xylose in the ratio of approximately 4:3, less amount of D-galactose, and also can contain L-fucose and L-arabinose Penicillium canescens xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O tamarind seed xyloglucan Talaromyces verruculosus xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O tamarind seed xyloglucan Penicillium canescens xyloglucan oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.151 ? x * 25000, XG 25, SDS-PAGE, x * 70000, XG 70, SDS-PAGE, XG 25 and XG 70 are organized in an enzyme complex Talaromyces verruculosus
3.2.1.151 ? x * 25000, XG A, SDS-PAGE, x * 26154, XG A, sequence calculation Penicillium canescens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.151 More the enzyme belongs to the glycohydrolase family 12 Talaromyces verruculosus
3.2.1.151 More the enzyme belongs to the glycohydrolase family 12 Penicillium canescens
3.2.1.151 XG 25
-
 Talaromyces verruculosus
3.2.1.151 XG 70
-
 Talaromyces verruculosus
3.2.1.151 XG A
-
 Penicillium canescens
3.2.1.151 xyloglucanase
-
 Talaromyces verruculosus
3.2.1.151 xyloglucanase A
-
 Penicillium canescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.151 50
-
 assay at Talaromyces verruculosus
3.2.1.151 50
-
 assay at Penicillium canescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.151 5
-
 assay at Talaromyces verruculosus
3.2.1.151 5
-
 assay at Penicillium canescens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.151 Talaromyces verruculosus XG 70
-
 3.5
3.2.1.151 Penicillium canescens XG A
-
 3.5
3.2.1.151 Talaromyces verruculosus XG 25
-
 4.1
3.2.1.151 Penicillium canescens XG A, sequence calculation
-
 4.96