Literature summary extracted from

Wei, J.; Kang, H.W.; Cohen, D.E.
Thioesterase superfamily member 2 (Them2)/acyl-CoA thioesterase 13 (Acot13): a homotetrameric hotdog fold thioesterase with selectivity for long-chain fatty acyl-CoAs (2009), Biochem. J., 421, 311-322.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.2.20	His-tagged version expressed in Escherichia coli BL21(DE3)	Mus musculus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.1.2.20	0.0073	-	myristoyl-CoA	at 25°C	Mus musculus
3.1.2.20	0.01	-	palmitoyl-CoA	-	Mus musculus
3.1.2.20	0.0106	-	myristoyl-CoA	at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5	Mus musculus
3.1.2.20	0.0136	-	myristoyl-CoA	at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0	Mus musculus
3.1.2.20	0.0155	-	myristoyl-CoA	at 37°C	Mus musculus
3.1.2.20	0.0159	-	palmitoyl-CoA	in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0	Mus musculus
3.1.2.20	0.018	-	palmitoyl-CoA	in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5	Mus musculus
3.1.2.20	0.0214	-	myristoyl-CoA	at 50°C	Mus musculus
3.1.2.20	0.0228	-	myristoyl-CoA	at 37°C, in the presence of small unilamellar vesicles composed of phosphatidylcholine at 4 mM total phospholipid concentration	Mus musculus
3.1.2.20	0.0274	-	oleoyl-CoA	-	Mus musculus
3.1.2.20	0.0275	-	lauroyl-CoA	-	Mus musculus
3.1.2.20	0.0472	-	decanoyl-CoA	-	Mus musculus
3.1.2.20	0.1382	-	hexanoyl-CoA	-	Mus musculus
3.1.2.20	0.1423	-	malonyl-CoA	-	Mus musculus
3.1.2.20	0.1628	-	3-hydroxybutyryl-CoA	-	Mus musculus
3.1.2.20	0.1916	-	phenylacetyl-CoA	-	Mus musculus
3.1.2.20	0.3057	-	3-hydroxy-3-methylglutaryl-CoA	-	Mus musculus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.1.2.20	additional information	optimal range for ionic strength is 80-160 mM for both NaCl and KCl	Mus musculus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.1.2.20	15000	-	4 * 15000, gel filtration	Mus musculus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.2.20	Mus musculus	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.1.2.20	brown adipose tissue	-	Mus musculus	-
3.1.2.20	heart	-	Mus musculus	-
3.1.2.20	kidney	-	Mus musculus	-
3.1.2.20	liver	-	Mus musculus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.1.2.20	0.0069	-	substrate 3-hydroxy-3-methylglutaryl-CoA	Mus musculus
3.1.2.20	0.014	-	substrate malonyl-CoA	Mus musculus
3.1.2.20	0.0298	-	substrate hexanoyl-CoA	Mus musculus
3.1.2.20	0.0304	-	substrate myristoyl-CoA, at 25°C	Mus musculus
3.1.2.20	0.0322	-	substrate myristoyl-CoA, at 37°C, in the presence of small unilamellar vesicles composed of phosphatidylcholine at 4 mM total phospholipid concentration	Mus musculus
3.1.2.20	0.0377	-	substrate lauroyl-CoA	Mus musculus
3.1.2.20	0.0381	-	substrate palmitoyl-CoA	Mus musculus
3.1.2.20	0.0454	-	substrate 3-hydroxybutyryl-CoA	Mus musculus
3.1.2.20	0.0469	-	substrate myristoyl-CoA, at 37°C	Mus musculus
3.1.2.20	0.0543	-	substrate decanoyl-CoA	Mus musculus
3.1.2.20	0.0568	-	substrate palmitoyl-CoA, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5	Mus musculus
3.1.2.20	0.0604	-	substrate oleoyl-CoA	Mus musculus
3.1.2.20	0.0614	-	substrate myristoyl-CoA, at 50°C	Mus musculus
3.1.2.20	0.0652	-	substrate palmitoyl-CoA, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0	Mus musculus
3.1.2.20	0.0681	-	substrate myristoyl-CoA, at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5	Mus musculus
3.1.2.20	0.0846	-	substrate myristoyl-CoA, at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0	Mus musculus
3.1.2.20	0.1198	-	substrate phenylacetyl-CoA	Mus musculus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.2.20	3-hydroxy-3-methylglutaryl-CoA + H2O	-	Mus musculus	CoA + 3-hydroxy-3-methylglutarate	-	?
3.1.2.20	3-hydroxybutyryl-CoA + H2O	-	Mus musculus	CoA + 3-hydroxybutyrate	-	?
3.1.2.20	decanoyl-CoA + H2O	-	Mus musculus	CoA + decanoate	-	?
3.1.2.20	hexanoyl-CoA + H2O	-	Mus musculus	CoA + hexanoate	-	?
3.1.2.20	lauroyl-CoA + H2O	-	Mus musculus	CoA + laurate	-	?
3.1.2.20	malonyl-CoA + H2O	-	Mus musculus	CoA + malonate	-	?
3.1.2.20	myristoyl-CoA + H2O	-	Mus musculus	CoA + myristate	-	?
3.1.2.20	oleoyl-CoA + H2O	-	Mus musculus	CoA + oleate	-	?
3.1.2.20	palmitoyl-CoA + H2O	-	Mus musculus	CoA + palmitate	-	?
3.1.2.20	phenylacetyl-CoA + H2O	-	Mus musculus	CoA + phenylacetate	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.1.2.20	homotetramer	4 * 15000, gel filtration	Mus musculus

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.2.20	ACOT13	-	Mus musculus
3.1.2.20	acyl-CoA thioesterase 13	-	Mus musculus
3.1.2.20	Them2	-	Mus musculus
3.1.2.20	thioesterase superfamily member 2	-	Mus musculus

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
3.1.2.20	additional information	-	Tm 59.2°C (transition beginning at temperatures in excess of 50°C)	Mus musculus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.1.2.20	additional information	-	additional information	inactive with acetyl-CoA and 2-butenoyl-CoA	Mus musculus
3.1.2.20	0.0066	-	palmitoyl-CoA	in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0	Mus musculus
3.1.2.20	0.007	-	3-hydroxy-3-methylglutaryl-CoA	-	Mus musculus
3.1.2.20	0.014	-	malonyl-CoA	-	Mus musculus
3.1.2.20	0.03	-	hexanoyl-CoA	-	Mus musculus
3.1.2.20	0.031	-	myristoyl-CoA	at 25°C	Mus musculus
3.1.2.20	0.033	-	myristoyl-CoA	at 37°C, in the presence of small unilamellar vesicles composed of phosphatidylcholine at 4 mM total phospholipid concentration	Mus musculus
3.1.2.20	0.038	-	palmitoyl-CoA	-	Mus musculus
3.1.2.20	0.038	-	lauroyl-CoA	-	Mus musculus
3.1.2.20	0.046	-	3-hydroxybutyryl-CoA	-	Mus musculus
3.1.2.20	0.047	-	myristoyl-CoA	at 37°C	Mus musculus
3.1.2.20	0.055	-	decanoyl-CoA	-	Mus musculus
3.1.2.20	0.057	-	palmitoyl-CoA	in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5	Mus musculus
3.1.2.20	0.061	-	oleoyl-CoA	-	Mus musculus
3.1.2.20	0.062	-	myristoyl-CoA	at 50°C	Mus musculus
3.1.2.20	0.069	-	myristoyl-CoA	at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5	Mus musculus
3.1.2.20	0.086	-	myristoyl-CoA	at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0	Mus musculus
3.1.2.20	0.12	-	phenylacetyl-CoA	-	Mus musculus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.1.2.20	7.2	9	-	Mus musculus

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3.1.2.20	0.023	-	3-hydroxy-3-methylglutaryl-CoA	-	Mus musculus
3.1.2.20	0.1	-	malonyl-CoA	-	Mus musculus
3.1.2.20	0.22	-	hexanoyl-CoA	-	Mus musculus
3.1.2.20	0.28	-	3-hydroxybutyryl-CoA	-	Mus musculus
3.1.2.20	0.63	-	phenylacetyl-CoA	-	Mus musculus
3.1.2.20	1.2	-	decanoyl-CoA	-	Mus musculus
3.1.2.20	1.4	-	lauroyl-CoA	-	Mus musculus
3.1.2.20	1.4	-	myristoyl-CoA	at 37°C, in the presence of small unilamellar vesicles composed of phosphatidylcholine at 4 mM total phospholipid concentration	Mus musculus
3.1.2.20	2	-	oleoyl-CoA	-	Mus musculus
3.1.2.20	2.9	-	myristoyl-CoA	at 50°C	Mus musculus
3.1.2.20	3	-	palmitoyl-CoA	-	Mus musculus
3.1.2.20	3	-	palmitoyl-CoA	in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5	Mus musculus
3.1.2.20	3.1	-	myristoyl-CoA	at 37°C	Mus musculus
3.1.2.20	4	-	palmitoyl-CoA	in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0	Mus musculus
3.1.2.20	4.4	-	myristoyl-CoA	at 25°C	Mus musculus
3.1.2.20	6	-	myristoyl-CoA	at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5	Mus musculus
3.1.2.20	6	-	myristoyl-CoA	at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0	Mus musculus

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Release 2023.1 (January 2023)