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Literature summary extracted from

  • Zhang, S.; Zhang, K.; Chen, X.; Chu, X.; Sun, F.; Dong, Z.
    Five mutations in N-terminus confer thermostability on mesophilic xylanase (2010), Biochem. Biophys. Res. Commun., 395, 200-206.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expression of wild-type and mutant AnxBs in Escherichia coli strain BL21 (DE3) Aspergillus niger
3.2.1.8 expression of wild-type and mutant SlxBs in Escherichia coli strain BL21 (DE3) Thermobifida fusca
3.2.1.8 expression of wild-type and mutant SoxBs in Escherichia coli strain BL21 (DE3) Streptomyces olivaceoviridis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.8 G23A/G24P/S26T the mutation increases the enzyme's thermostability Aspergillus niger
3.2.1.8 G23A/G24P/S26T the mutation increases the enzyme's thermostability Thermobifida fusca
3.2.1.8 G23A/G24P/S26T the mutation increases the enzyme's thermostability Streptomyces olivaceoviridis
3.2.1.8 additional information construction of an N-terminal replacement mutant Aspergillus niger
3.2.1.8 additional information construction of an N-terminal replacement mutant Thermobifida fusca
3.2.1.8 additional information substituting of Streptomyces olivaceovirdis SoxB 33 N-terminal amino acid residues with the corresponding residues of the thermophilic xylanase TfxA from Thermomonospora fusca strain ATCC 27730 significantly enhances the enzyme thermostability, overview Streptomyces olivaceoviridis
3.2.1.8 T11Y/N12H/N13D/F15Y/F16F the mutation increases the enzyme's thermostability Aspergillus niger
3.2.1.8 T11Y/N12H/N13D/F15Y/F16F the mutation increases the enzyme's thermostability Thermobifida fusca
3.2.1.8 T11Y/N12H/N13D/F15Y/F16F the mutation increases the enzyme's thermostability Streptomyces olivaceoviridis
3.2.1.8 T30E/N32G/S33P the mutation increases the enzyme's thermostability Aspergillus niger
3.2.1.8 T30E/N32G/S33P the mutation increases the enzyme's thermostability Thermobifida fusca
3.2.1.8 T30E/N32G/S33P the mutation increases the enzyme's thermostability Streptomyces olivaceoviridis
3.2.1.8 V3A/I4V/T6S/Q8E the mutation increases the enzyme's thermostability Aspergillus niger
3.2.1.8 V3A/I4V/T6S/Q8E the mutation increases the enzyme's thermostability Thermobifida fusca
3.2.1.8 V3A/I4V/T6S/Q8E the mutation increases the enzyme's thermostability Streptomyces olivaceoviridis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Aspergillus niger
-
-
-
3.2.1.8 Aspergillus niger UV-11
-
-
-
3.2.1.8 Streptomyces olivaceoviridis
-
 strain ATCC 23630
-
3.2.1.8 Thermobifida fusca
-
 strain ATCC 27730
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 additional information
-
 activities of wild-type and mutant enzymes, overview Aspergillus niger
3.2.1.8 additional information
-
 activities of wild-type and mutant enzymes, overview Thermobifida fusca
3.2.1.8 additional information
-
 activities of wild-type and mutant enzymes, overview Streptomyces olivaceoviridis
3.2.1.8 427
-
 purified recombinant wild-type SlxB Thermobifida fusca
3.2.1.8 801
-
 purified recombinant wild-type SoxB Streptomyces olivaceoviridis
3.2.1.8 1342
-
 purified recombinant wild-type AnxB Aspergillus niger

Subunits

EC Number Subunits Comment Organism
3.2.1.8 More enzyme N-terminal structure analysis and homology modelling, overview Aspergillus niger
3.2.1.8 More enzyme N-terminal structure analysis and homology modelling, overview Thermobifida fusca
3.2.1.8 More enzyme N-terminal structure analysis and homology modelling, overview Streptomyces olivaceoviridis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 AnxB
-
 Aspergillus niger
3.2.1.8 More the enzyme belongs to the glycosyl hydrolase family 11, GH11 Aspergillus niger
3.2.1.8 More the enzyme belongs to the glycosyl hydrolase family 11, GH11 Thermobifida fusca
3.2.1.8 More the enzyme belongs to the glycosyl hydrolase family 11, GH11 Streptomyces olivaceoviridis
3.2.1.8 SlxB
-
 Thermobifida fusca
3.2.1.8 SoxB
-
 Streptomyces olivaceoviridis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50
-
-
 Aspergillus niger
3.2.1.8 55
-
-
 Thermobifida fusca
3.2.1.8 65
-
-
 Streptomyces olivaceoviridis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6
-
 assay at Aspergillus niger
3.2.1.8 6
-
 assay at Thermobifida fusca
3.2.1.8 6
-
 assay at Streptomyces olivaceoviridis