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Literature summary extracted from

  • Jamaluddin, H.; Tumbale, P.; Ferns, T.A.; Thiyagarajan, N.; Brew, K.; Acharya, K.R.
    Crystal structure of alpha-1,3-galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNPbetaGal) (2009), Biochem. Biophys. Res. Commun., 385, 601-604.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.87 synthesis attaching beta p-nitrophenyl to galactose converts the complex from a poor into a good substrate, the group mimics a monosaccharide like N-acetylglucosamine Bos taurus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.87 5% PEG 6000, 0.1 M Tris-HCl, pH 8.0, 10 mM MnCl2, 10 mM UDP, in complex with substrate p-nitrophenyl-beta-galactoside, 25% glycerol as cryoprotectant Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.87 additional information attaching beta p-nitrophenyl to galactose converts the complex from a poor into a good substrate, the group mimics a monosaccharide like N-acetylglucosamine Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.87 0.6
-
N-acetyllactosamine 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
2.4.1.87 1
-
p-nitrophenyl-beta-D-galactoside 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
2.4.1.87 107
-
p-nitrophenyl-alpha-D-galactoside 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
2.4.1.87 392
-
D-galactose 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.87 MnCl2
-
Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.87 UDP-alpha-D-galactose + N-acetyllactosamine Bos taurus
-
UDP + ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.87 Bos taurus P14769
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.87
-
Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.87 UDP-alpha-D-galactose + N-acetyllactosamine
-
Bos taurus UDP + ?
-
?
2.4.1.87 UDP-galactose + D-galactose
-
Bos taurus UDP + alpha-D-galactosyl-1,3-D-galactoside
-
?
2.4.1.87 UDP-galactose + N-acetyllactosamine
-
Bos taurus UDP + ?
-
?
2.4.1.87 UDP-galactose + p-nitrophenyl-alpha-D-galactoside
-
Bos taurus UDP + ?
-
?
2.4.1.87 UDP-galactose + p-nitrophenyl-beta-D-galactoside
-
Bos taurus UDP + ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.87 alpha-1,3-galactosyltransferase
-
Bos taurus
2.4.1.87 alpha3GT
-
Bos taurus
2.4.1.87 UDP-galactose beta-galactosyl alpha-1,3-galactosyltransferase
-
Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.87 0.059
-
p-nitrophenyl-alpha-D-galactoside 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
2.4.1.87 0.32
-
D-galactose 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
2.4.1.87 1.1
-
p-nitrophenyl-beta-D-galactoside 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
2.4.1.87 1.9
-
N-acetyllactosamine 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus

General Information

EC Number General Information Comment Organism
2.4.1.87 metabolism synthesis of biologically active oligosaccharides Bos taurus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.87 0.0006
-
p-nitrophenyl-alpha-D-galactoside 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
2.4.1.87 0.0008
-
D-galactose 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
2.4.1.87 1.1
-
p-nitrophenyl-beta-D-galactoside 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus
2.4.1.87 3.2
-
N-acetyllactosamine 10 mM MnCl2, 0.3 mM UDP-galactose Bos taurus