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Literature summary extracted from

  • Wright, M.H.; Farquhar, M.J.; Aletrari, M.O.; Ladds, G.; Hodgkin, M.N.
    Identification of caspase 3 motifs and critical aspartate residues in human phospholipase D1beta and phospholipase D2alpha (2008), Biochem. Biophys. Res. Commun., 369, 478-484.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 Phorbol esters activate isozyme PLD1beta Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.4 expression of isozyme PLD2alpha in HEK-293 cells, overexpression of GFP-tagged isozyme in vitro Homo sapiens
3.1.4.4 expression of isozymes PLD1beta and PLD2alpha in HEK-293 cells, overexpression of GFP-tagged isozymes in vitro Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.4 D16A caspase 3 cleavage motif mutant of isozyme PLD2 Homo sapiens
3.1.4.4 D16A site-directed mutagenesis of isozyme PLD2D16A, the mutant shows altered cleavage by caspase 3 compared to the wild-type enzyme Homo sapiens
3.1.4.4 D28A caspase 3 cleavage motif mutant of isozyme PLD2 Homo sapiens
3.1.4.4 D28A site-directed mutagenesis of isozyme PLD2D28A, the mutant does not show altered cleavage by caspase 3 compared to the wild-type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 additional information isozyme PLD2alpha contains functional caspase 3 cleavage sites; isozymes PLD1beta and PLD2alpha contain functional caspase 3 cleavage sites and identify the critical aspartate residues within PLD1beta that affect its activation by phorbol esters and attenuate phosphatidylcholine hydrolysis during apoptosis Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.4 plasma membrane
-
 Homo sapiens 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 additional information Homo sapiens PLD isozymes are cleaved by caspase 3, cleavage site determination, isozyme PLD2alpha contains two consensus motifs for caspase 3 cleavage, DXXD or D/E, D/E, X, D, located in the loop region at DDVD545S between the PLD domains, mutational analysis, overview ?
-
 ?
3.1.4.4 additional information Homo sapiens PLD isozymes are cleaved by caspase 3, cleavage site determination, isozymes PLD1beta and PLD2alpha contain each two consensus motifs for caspase 3 cleavage, DXXD or D/E, D/E, X, D, located in the loop region at DDVD545S and DFID631R between the PLD domains, respectively, mutational analysis, overview ?
-
 ?
3.1.4.4 phosphatidylcholine + H2O Homo sapiens
-
 choline + phosphatidic acid phosphatidic acid plays a regulatory role in important cellular processes such as secretion, cellular shape change, and movement ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Homo sapiens O14939 PLD2; isozyme PLD2alpha
-
3.1.4.4 Homo sapiens Q13393 PLD1; isozymes PLD1beta and PLD2alpha
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 additional information PLD isozymes are cleaved by caspase 3, cleavage site determination, isozyme PLD2alpha contains two consensus motifs for caspase 3 cleavage, DXXD or D/E, D/E, X, D, located in the loop region at DDVD545S between the PLD domains, mutational analysis, overview Homo sapiens ?
-
 ?
3.1.4.4 additional information PLD isozymes are cleaved by caspase 3, cleavage site determination, isozymes PLD1beta and PLD2alpha contain each two consensus motifs for caspase 3 cleavage, DXXD or D/E, D/E, X, D, located in the loop region at DDVD545S and DFID631R between the PLD domains, respectively, mutational analysis, overview Homo sapiens ?
-
 ?
3.1.4.4 additional information PLD also shows transphosphatidylation activity with 1-butanol resulting in formation of phosphatidylbutanol Homo sapiens ?
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Homo sapiens choline + phosphatidic acid
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Homo sapiens choline + phosphatidic acid phosphatidic acid plays a regulatory role in important cellular processes such as secretion, cellular shape change, and movement ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 phospholipase D1beta
-
 Homo sapiens
3.1.4.4 phospholipase D2alpha
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
3.1.4.4 physiological function phosphatidic acid plays a regulatory role in important cellular processes such as secretion, cellular shape change, and movement. PLD-based signaling also plays a pro-mitogenic and pro-survival role in cells and therefore anti-apoptotic Homo sapiens