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Literature summary extracted from

  • Habe, H.; Morii, K.; Fushinobu, S.; Nam, J.W.; Ayabe, Y.; Yoshida, T.; Wakagi, T.; Yamane, H.; Nojiri, H.; Omori, T.
    Crystal structure of a histidine-tagged serine hydrolase involved in the carbazole degradation (CarC enzyme) (2003), Biochem. Biophys. Res. Commun., 303, 631-639.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.13 expression in Escherichia coli Pseudomonas resinovorans
3.7.1.13 expression in Escherichia coli Janthinobacterium sp. J3

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.7.1.13 to 1.86 A resolution, space group I422. The subunit of ht-CarCJ3 is divided into two domains, i.e., the core domain with residues 15-144 and 209-282, and the lid domain with residues 145-208. The invisible 10 amino acid residues Ile146-Asn155 correspond to the helix alpha4, which is the putative first alpha-helix in the lid domain. Sidechains of the active-site residues, Ser114 and His261, are clearly shown in the electron density map Janthinobacterium sp. J3

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.13 0.00251
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.5, 25°C Pseudomonas resinovorans
3.7.1.13 0.00273
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.5, 25°C Janthinobacterium sp. J3

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.13 33000
-
2 * 33000, SDS-PAGE Pseudomonas resinovorans
3.7.1.13 33000
-
2 * 33000, SDS-PAGE Janthinobacterium sp. J3
3.7.1.13 70000
-
gel filtration Pseudomonas resinovorans
3.7.1.13 70000
-
gel filtration Janthinobacterium sp. J3

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.13 Janthinobacterium sp. J3 Q84II3
-
-
3.7.1.13 Pseudomonas resinovorans
-
-
-
3.7.1.13 Pseudomonas resinovorans CA10
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.13 recombinant enzyme Janthinobacterium sp. J3
3.7.1.13 recombinantv enzyme Pseudomonas resinovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.13 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
Pseudomonas resinovorans ?
-
?
3.7.1.13 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
Janthinobacterium sp. J3 ?
-
?
3.7.1.13 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
Pseudomonas resinovorans CA10 ?
-
?
3.7.1.13 2-hydroxy-6-oxohepta-2,4-dienoic acid + H2O 0.38% of the activity with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Pseudomonas resinovorans ?
-
?
3.7.1.13 2-hydroxy-6-oxohepta-2,4-dienoic acid + H2O 0.56% of the activity with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Janthinobacterium sp. J3 ?
-
?
3.7.1.13 2-hydroxy-6-oxohepta-2,4-dienoic acid + H2O 0.38% of the activity with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Pseudomonas resinovorans CA10 ?
-
?
3.7.1.13 2-hydroxymuconic semialdehyde + H2O 0.49% of the activity with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Janthinobacterium sp. J3 ?
-
?
3.7.1.13 2-hydroxymuconic semialdehyde + H2O 0.54% of the activity with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Pseudomonas resinovorans ?
-
?
3.7.1.13 2-hydroxymuconic semialdehyde + H2O 0.54% of the activity with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Pseudomonas resinovorans CA10 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.7.1.13 dimer 2 * 33000, SDS-PAGE Pseudomonas resinovorans
3.7.1.13 dimer 2 * 33000, SDS-PAGE Janthinobacterium sp. J3

Synonyms

EC Number Synonyms Comment Organism
3.7.1.13 CarC
-
Pseudomonas resinovorans
3.7.1.13 CarC
-
Janthinobacterium sp. J3

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7.1.13 1.99
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.5, 25°C Janthinobacterium sp. J3
3.7.1.13 2.14
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.5, 25°C Pseudomonas resinovorans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.7.1.13 730
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.5, 25°C Janthinobacterium sp. J3
3.7.1.13 852
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.5, 25°C Pseudomonas resinovorans