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Literature summary extracted from

  • Lee, M.; Kim, Y.-W.; Kim, T.-J.; Park, C.-S.; Kim, J.-W.; Moon, T.-W.; Park, K.-H.
    A novel amylolytic enzyme from Thermotoga maritima, resembling cyclodextrinase and alpha-glucosidase, that liberates glucose from the reducing end of the substrates (2002), Biochem. Biophys. Res. Commun., 295, 818-825.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B8 gene TM1835, expression in Escherichia coli strain MC1061 Thermotoga maritima MSB8

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B8 Ca2+
-
 Thermotoga maritima MSB8
3.2.1.B8 Co2+
-
 Thermotoga maritima MSB8
3.2.1.B8 Fe2+
-
 Thermotoga maritima MSB8
3.2.1.B8 Mg2+
-
 Thermotoga maritima MSB8
3.2.1.B8 Mn2+
-
 Thermotoga maritima MSB8
3.2.1.B8 additional information no inhibition by EGTA and EDTA Thermotoga maritima MSB8
3.2.1.B8 Zn2+
-
 Thermotoga maritima MSB8

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B8 2.26
-
 4-nitrophenyl alpha-maltopentaoside pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 7.41
-
 maltoheptaose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 7.77
-
 maltohexaose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 9.95
-
 maltopentaose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 11.84
-
 maltotetraose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 14.53
-
 beta-cyclodextrin pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 20.64
-
 maltotriose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.B8 acarbose + 2 H2O Thermotoga maritima MSB8
-
 acarviosine + 2 D-glucose
-
 ?
3.2.1.B8 alpha-cyclodextrin + H2O Thermotoga maritima MSB8
-
 maltose + D-glucose
-
 ?
3.2.1.B8 beta-cyclodextrin + H2O Thermotoga maritima MSB8
-
 maltose + D-glucose
-
 ?
3.2.1.B8 gamma-cyclodextrin + H2O Thermotoga maritima MSB8
-
 maltose + D-glucose
-
 ?
3.2.1.B8 maltoheptaose + H2O Thermotoga maritima MSB8
-
 maltose + D-glucose + ?
-
 ?
3.2.1.B8 maltohexaose + H2O Thermotoga maritima MSB8
-
 maltose + D-glucose + ?
-
 ?
3.2.1.B8 maltopentaose + H2O Thermotoga maritima MSB8
-
 maltose + D-glucose + ?
-
 ?
3.2.1.B8 maltotetraose + H2O Thermotoga maritima MSB8
-
 maltose + 2 D-glucose
-
 ?
3.2.1.B8 maltotriose + H2O Thermotoga maritima MSB8
-
 maltose + D-glucose
-
 ?
3.2.1.B8 additional information Thermotoga maritima MSB8 TMG is an exo-acting glucosidase possessing the characteristics of both CD-/pullulan hydrolyzing enzyme and alpha-glucosidase. It hydrolyzes various maltodextrins including maltotriose to maltoheptaose and cyclomaltodextrins to mainly glucose and maltose, but it cannot hydrolyze pullulan, but acarbose ?
-
 ?
3.2.1.B8 soluble starch + H2O Thermotoga maritima MSB8
-
 maltose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B8 Thermotoga maritima MSB8 Q9X2F4 gene TM1835; gene TM1835
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B8 recombinant TMG 18.2fold from Escherichia coli strain MC1061 by heat treatment, hydrophobic interaction and anion exchange chromatography Thermotoga maritima MSB8

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.B8 0.24
-
 purified recombinant enzyme, substrate soluble starch Thermotoga maritima MSB8
3.2.1.B8 4.85
-
 purified recombinant enzyme, substrate beta-cyclodextrin Thermotoga maritima MSB8
3.2.1.B8 18.6
-
 purified recombinant enzyme, substrate maltotriose Thermotoga maritima MSB8

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B8 4-nitrophenyl alpha-maltopentaoside
-
 Thermotoga maritima MSB8 maltopentaose + 4-nitrophenol
-
 ?
3.2.1.B8 4-nitrophenyl alpha-maltopentaoside + H2O
-
 Thermotoga maritima MSB8 maltopentaose + 4-nitrophenol further hydrolysis of maltopentaose by TMG ?
3.2.1.B8 acarbose + 2 H2O
-
 Thermotoga maritima MSB8 acarviosine + 2 D-glucose
-
 ?
3.2.1.B8 alpha-cyclodextrin + H2O
-
 Thermotoga maritima MSB8 maltose + D-glucose
-
 ?
3.2.1.B8 beta-cyclodextrin + H2O
-
 Thermotoga maritima MSB8 maltose + D-glucose
-
 ?
3.2.1.B8 gamma-cyclodextrin + H2O
-
 Thermotoga maritima MSB8 maltose + D-glucose
-
 ?
3.2.1.B8 malto-heptaose + H2O
-
 Thermotoga maritima MSB8 maltose + D-glucose
-
 ?
3.2.1.B8 malto-hexaose + H2O
-
 Thermotoga maritima MSB8 maltose + D-glucose
-
 ?
3.2.1.B8 malto-pentaose + H2O
-
 Thermotoga maritima MSB8 maltose + D-glucose
-
 ?
3.2.1.B8 malto-tetraose + H2O
-
 Thermotoga maritima MSB8 maltose + D-glucose
-
 ?
3.2.1.B8 malto-triose + H2O
-
 Thermotoga maritima MSB8 maltose + D-glucose
-
 ?
3.2.1.B8 maltoheptaose + H2O
-
 Thermotoga maritima MSB8 maltose + D-glucose + ?
-
 ?
3.2.1.B8 maltohexaose + H2O
-
 Thermotoga maritima MSB8 maltose + D-glucose + ?
-
 ?
3.2.1.B8 maltopentaose + H2O
-
 Thermotoga maritima MSB8 maltose + D-glucose + ?
-
 ?
3.2.1.B8 maltotetraose + H2O
-
 Thermotoga maritima MSB8 maltose + 2 D-glucose
-
 ?
3.2.1.B8 maltotriose + H2O
-
 Thermotoga maritima MSB8 maltose + D-glucose
-
 ?
3.2.1.B8 additional information TMG is an exo-acting glucosidase possessing the characteristics of both CD-/pullulan hydrolyzing enzyme and alpha-glucosidase. It hydrolyzes various maltodextrins including maltotriose to maltoheptaose and cyclomaltodextrins to mainly glucose and maltose, but it cannot hydrolyze pullulan, but acarbose Thermotoga maritima MSB8 ?
-
 ?
3.2.1.B8 additional information the enzyme specifically cleaves a glucose unit from the reducing end of maltooligosaccharides unlike to other glucosidases. Since its enzymatic activity is negligible if alpha-methylglucoside is present in the reducing end, the type of the residue at the reducing end of the substrate is important for the TMG activity. It hydrolyzes various maltodextrins including maltotriose to maltoheptaose and cyclomaltodextrins to mainly glucose and maltose, but it cannot hydrolyze pullulan, but acarbose Thermotoga maritima MSB8 ?
-
 ?
3.2.1.B8 soluble starch + H2O
-
 Thermotoga maritima MSB8 maltose + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B8 cyclodextrinase
-
 Thermotoga maritima MSB8
3.2.1.B8 TMG
-
 Thermotoga maritima MSB8

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B8 85
-
 assay at Thermotoga maritima MSB8

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.B8 60 95
-
 Thermotoga maritima MSB8

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B8 1.37
-
 beta-cyclodextrin pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 3.2
-
 maltotriose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 4.09
-
 maltoheptaose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 4.34
-
 maltotetraose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 4.56
-
 maltohexaose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 5.18
-
 maltopentaose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 16.28
-
 4-nitrophenyl alpha-maltopentaoside pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B8 6.5
-
 assay at Thermotoga maritima MSB8

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B8 4 8
-
 Thermotoga maritima MSB8

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B8 0.094
-
 beta-cyclodextrin pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 0.16
-
 maltotriose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 0.37
-
 maltotetraose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 0.52
-
 maltopentaose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 0.55
-
 maltoheptaose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 0.59
-
 maltohexaose pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8
3.2.1.B8 7.2
-
 4-nitrophenyl alpha-maltopentaoside pH 6.5, 85°C, recombinant enzyme Thermotoga maritima MSB8