Any feedback?
Literature summary extracted from
- Unsaturated glucuronyl hydrolase of Bacillus sp. GL1: novel enzyme prerequisite for metabolism of unsaturated oligosaccharides produced by polysaccharide lyases (1999), Arch. Biochem. Biophys., 368, 367-374.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.179 | expression in Escherichia coli | Bacillus sp. (in: Bacteria) |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.179 | Ca2+ | 1 mM, 46% inhibition | Bacillus sp. (in: Bacteria) |  |
| 3.2.1.179 | Co2+ | 1 mM, 22% inhibition | Bacillus sp. (in: Bacteria) | |
| 3.2.1.179 | Cu2+ | 1 mM, 69% inhibition | Bacillus sp. (in: Bacteria) | |
| 3.2.1.179 | Fe2+ | 1 mM, 94% inhibition | Bacillus sp. (in: Bacteria) | |
| 3.2.1.179 | Hg2+ | 1 mM, 77% inhibition | Bacillus sp. (in: Bacteria) | |
| 3.2.1.179 | Zn2+ | 1 mM, 21% ihibition | Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.179 | 42000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O | Bacillus sp. (in: Bacteria) | degradation of oligosaccharides with unsaturated uronic acid at the nonreducing terminal produced by polysaccharide lyases | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | - |
? | |
| 3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O | Bacillus sp. (in: Bacteria) GL1 | degradation of oligosaccharides with unsaturated uronic acid at the nonreducing terminal produced by polysaccharide lyases | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.179 | Bacillus sp. (in: Bacteria) | - |
- |
- |
| 3.2.1.179 | Bacillus sp. (in: Bacteria) GL1 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.179 | - |
Bacillus sp. (in: Bacteria) |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.179 | culture condition:gellan-grown cell | - |
Bacillus sp. (in: Bacteria) | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O | 114% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | Bacillus sp. (in: Bacteria) | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-GalNAc | - |
? | |
| 3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc + H2O | 114% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | Bacillus sp. (in: Bacteria) GL1 | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-GalNAc | - |
? | |
| 3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O | 25.5% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | Bacillus sp. (in: Bacteria) | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-GalNAc6S | - |
? | |
| 3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GalNAc6S + H2O | 25.5% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | Bacillus sp. (in: Bacteria) GL1 | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-GalNAc6S | - |
? | |
| 3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->3)-beta-D-GlcNAc + H2O | 116% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | Bacillus sp. (in: Bacteria) | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-GlcNAc | - |
? | |
| 3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O | - |
Bacillus sp. (in: Bacteria) | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | - |
? | |
| 3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O | degradation of oligosaccharides with unsaturated uronic acid at the nonreducing terminal produced by polysaccharide lyases | Bacillus sp. (in: Bacteria) | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | - |
? | |
| 3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O | - |
Bacillus sp. (in: Bacteria) GL1 | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | - |
? | |
| 3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O | degradation of oligosaccharides with unsaturated uronic acid at the nonreducing terminal produced by polysaccharide lyases | Bacillus sp. (in: Bacteria) GL1 | 4-deoxy-L-threo-5-hexosulose-uronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | - |
? | |
| 3.2.1.179 | beta-D-4-deoxy-DELTA4,5-GlcAp2S-(1->3)-beta-D-GalNAc + H2O | 23.5% of the activity with beta-D-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp | Bacillus sp. (in: Bacteria) | ? + beta-D-GalNAc | - |
? | |
| 3.2.1.179 | additional information | the enzyme is specific for unsaturated glucuronides and inert to saturated glucuronides. The enzyme fails to liberate nitrophenol from p-nitrophenyl-beta-D-glucuronide. The enzyme prefers unsaturated chondroitin disaccharide without sulfate to those with sulfate | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3.2.1.179 | additional information | the enzyme is specific for unsaturated glucuronides and inert to saturated glucuronides. The enzyme fails to liberate nitrophenol from p-nitrophenyl-beta-D-glucuronide. The enzyme prefers unsaturated chondroitin disaccharide without sulfate to those with sulfate | Bacillus sp. (in: Bacteria) GL1 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.179 | monomer | 1 * 42000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.179 | 45 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.179 | 30 | 55 | 30°C: about 90% of maximal activity, 55°C: about 35% of maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.179 | 50 | - |
pH 7.0, 10 min, about 40% loss of activity | Bacillus sp. (in: Bacteria) |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.179 | 6 | 6.5 | sodium acetate buffer | Bacillus sp. (in: Bacteria) |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.2.1.179 | Bacillus sp. (in: Bacteria) | induced in the bacterial cells grown on either gellan or xanthan. Expression levels of unsaturated glucuronyl hydrolase in the bacterium grown on gellan and xanthan are 20.7 and 16.4 mU/mg protein, respectively, while the bacterium grown in the glucose, pectin, or LB medium fails to produce the enzyme | up |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
