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Literature summary extracted from

  • Abokitse, K.; Wu, M.; Bergeron, H.; Grosse, S.; Lau, P.C.
    Thermostable feruloyl esterase for the bioproduction of ferulic acid from triticale bran (2010), Appl. Microbiol. Biotechnol., 87, 195-203.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.73 industry bioproduction of ferulic acid from triticale bran by alkaline and thermostable FAE Caldanaerobacter subterraneus subsp. tengcongensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 777-bp DNA fragment digested with EcoRI and PstI and ligated into the corresponding sites of the IPTG-inducible plasmid pKK223-3, transformed into Escherichia coli strain JM109 and expressed from pKTtFAE vector Caldanaerobacter subterraneus subsp. tengcongensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.16
-
 methyl p-coumarate at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 0.29
-
 methyl sinapate at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 0.32
-
 methyl ferulate at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 29100
-
 sequence analysis Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 33000
-
 1 * 33000, SDS-PAGE, purified enzyme Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 36000
-
 native enzyme Caldanaerobacter subterraneus subsp. tengcongensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Caldanaerobacter subterraneus subsp. tengcongensis
-
-
-
3.1.1.73 Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 to apparent homogeneity by centrifugation, ammonium sulfate precipitation, ultrafiltration and gel filtration, 30.5fold with a yield of 9% Caldanaerobacter subterraneus subsp. tengcongensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 additional information
-
 with 5 mM methyl syringate as substrate only traces, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 0.0004
-
 with 1 mM p-nitrophenyl palmitate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 0.03
-
 with 5 mM methyl vanillate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 0.05
-
 with 5 mM methyl caffeate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 0.426
-
 with 5 mM methyl p-coumarate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 0.456
-
 with 5 mM methyl ferulate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 0.462
-
 with feruloylated oligosaccharides as substrate, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 0.511
-
 with 5 mM methyl sinapate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 1.7
-
 with 1 mM p-nitrophenyl myristate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 10.5
-
 with 1 mM p-nitrophenyl caprylate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 18.5
-
 with 1 mM p-nitrophenyl acetate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 36
-
 with 1 mM p-nitrophenyl butyrate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 feruloylated oligosaccharide + H2O ferulic acid is released from feruloylated oligosaccharides prepared from triticale bran. Recovery of 68 mg ferulic acid/100 g triticale bran by a 30% release of the total ferulic acid from triticale bran within a 5 h incubation period. Trichoderma viride xylanase combined with FAE can release 29.2 mg/100 g material Caldanaerobacter subterraneus subsp. tengcongensis ferulic acid + oligosaccharide
-
 ?
3.1.1.73 methyl caffeate + H2O poor substrate Caldanaerobacter subterraneus subsp. tengcongensis caffeic acid + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O poor substrate Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis ferulic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 ferulic acid + methanol
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis p-coumaric acid + methanol
-
 ?
3.1.1.73 methyl sinapate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis sinapic acid + methanol
-
 ?
3.1.1.73 methyl syringate + H2O poor substrate Caldanaerobacter subterraneus subsp. tengcongensis ? + methanol
-
 ?
3.1.1.73 methyl vanillate + H2O poor substrate Caldanaerobacter subterraneus subsp. tengcongensis ? + methanol
-
 ?
3.1.1.73 p-nitrophenyl acetate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis p-nitrophenol + acetic acid
-
 ?
3.1.1.73 p-nitrophenyl acetate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 p-nitrophenol + acetic acid
-
 ?
3.1.1.73 p-nitrophenyl butyrate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis p-nitrophenol + butyric acid
-
 ?
3.1.1.73 p-nitrophenyl butyrate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 p-nitrophenol + butyric acid
-
 ?
3.1.1.73 p-nitrophenyl caprylate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis p-nitrophenol + caprylate
-
 ?
3.1.1.73 p-nitrophenyl myristate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis p-nitrophenol + myristate
-
 ?
3.1.1.73 p-nitrophenyl palmitate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis p-nitrophenol + palmitate
-
 ?
3.1.1.73 p-nitrophenyl palmitate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 p-nitrophenol + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 monomer 1 * 33000, SDS-PAGE, purified enzyme Caldanaerobacter subterraneus subsp. tengcongensis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAE
-
 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 feruloyl esterase
-
 Caldanaerobacter subterraneus subsp. tengcongensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 75 80 at 75°C retains at least 95% of its original activity for over 80 min. At 80°C, its half-life is 50 min, rendering FAE a highly thermostable protein Caldanaerobacter subterraneus subsp. tengcongensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 0.22
-
 methyl p-coumarate at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 0.28
-
 methyl ferulate at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
3.1.1.73 0.28
-
 methyl sinapate at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 8
-
 purified enzyme, in 50 mM Britton and Robinson's universal buffer Caldanaerobacter subterraneus subsp. tengcongensis