Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shi, P.; Yao, G.; Yang, P.; Li, N.; Luo, H.; Bai, Y.; Wang, Y.; Yao, B.
    Cloning, characterization, and antifungal activity of an endo-1,3-beta-D-glucanase from Streptomyces sp. S27 (2010), Appl. Microbiol. Biotechnol., 85, 1483-1490.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.39 2-mercaptoethanol 9,6-10% activation at 1-5 mM Streptomyces sp.

Application

EC Number Application Comment Organism
3.2.1.39 agriculture BglS27 is a good candidate for utilization in biotechnological applications such as plant protection, feed, and food preservation Streptomyces sp.
3.2.1.39 biotechnology BglS27 is a good candidate for utilization in biotechnological applications such as plant protection, feed, and food preservation Streptomyces sp.
3.2.1.39 food industry BglS27 is a good candidate for utilization in biotechnological applications such as plant protection, feed, and food preservation Streptomyces sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 gene bglS27, DNA and amino acid sequence determination and analysis, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.39 Ca2+ loss of 11.5% activity at 5 mM Streptomyces sp.
3.2.1.39 Cr3+ loss of 11.6% activity at 5 mM Streptomyces sp.
3.2.1.39 Cu2+ loss of 24.4% activity at 5 mM Streptomyces sp.
3.2.1.39 EDTA
-
 Streptomyces sp.
3.2.1.39 Fe2+ loss of 22.6% activity at 5 mM Streptomyces sp.
3.2.1.39 Mn2+ loss of 24.2% activity at 5 mM Streptomyces sp.
3.2.1.39 Zn2+ loss of 22.0% activity at 5 mM Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 additional information
-
 additional information 1.89 mg/ml for laminarin, recombinant enzyme Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.39 Co2+ 14.1% activation at 1 mM, 30% at 5 mM Streptomyces sp.
3.2.1.39 Mn2+ 20.4% activation at 1 mM Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 42700
-
 x * 42700, about, sequence calculation Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.39 laminarin + H2O Streptomyces sp.
-
 ?
-
 ?
3.2.1.39 additional information Streptomyces sp. BglS27 preferentially catalyzes the hydrolysis of glucans with a beta-1,3-linkage using an endolytic mode of action ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Streptomyces sp. C3VPJ1 gene bglS27
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 recombinant His6-tagged BglS27 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 236
-
 purified recombinant enzyme, substrate laminarin Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 beta-glucan + H2O Barley beta-glucan, 1,3-1,4-beta-glucose monomers, high activity Streptomyces sp. ?
-
 ?
3.2.1.39 laminarin + H2O
-
 Streptomyces sp. ?
-
 ?
3.2.1.39 laminarin + H2O 1,3-beta-glucose monomers, best substrate Streptomyces sp. ?
-
 ?
3.2.1.39 lichenan + H2O 1,3-1,4-beta-glucose monomers, high activity Streptomyces sp. ?
-
 ?
3.2.1.39 additional information BglS27 preferentially catalyzes the hydrolysis of glucans with a beta-1,3-linkage using an endolytic mode of action Streptomyces sp. ?
-
 ?
3.2.1.39 additional information no activity with oat spelt xylan and carboxymethyl cellulose Streptomyces sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 42700, about, sequence calculation Streptomyces sp.
3.2.1.39 More the encoded protein comprises a catalytic module of glycosyl hydrolase family 16, a short glycine linker region, and a family 13 carbohydrate-binding module Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 BglS27
-
 Streptomyces sp.
3.2.1.39 endo-1,3-beta-D-glucanase
-
 Streptomyces sp.
3.2.1.39 More the bacterial enzyme belongs to glycosylhydrolase family GH16 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 65
-
 recombinant enzyme Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 60
-
 purified recombinant enzyme, over 80% activity remaining after 30 min Streptomyces sp.
3.2.1.39 70 80 purified recombinant enzyme, over 40% activity remaining after 30 min Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 5.5
-
 recombinant enzyme Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.39 5 9 activity range Streptomyces sp.

General Information

EC Number General Information Comment Organism
3.2.1.39 physiological function BglS27 has the ability to inhibit the growth of phytopathogenic fungi Rhizoctonic solani and Fusarium oxysporum and some mycotoxin-producing fungi Fusarium crookwellense and Paecilomyces variotii Streptomyces sp.