Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gupta, V.; Prasanna, R.; Natarajan, C.; Srivastava, A.K.; Sharma, J.
    Identification, characterization, and regulation of a novel antifungal chitosanase gene (cho) in Anabaena spp. (2010), Appl. Environ. Microbiol., 76, 2769-2777.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 gene cho, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 Trichormus fertilissimus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Trichormus fertilissimus C9D7S2 RPAN1, gene cho
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan pentasaccharide + H2O hydrolysis of the chitosan oligosaccharide (GlcN)5 to (GlcN)2 and (GlcN)3 Trichormus fertilissimus chitosan disaccharide + chitosan trisaccharide
-
 ?
3.2.1.132 additional information catalytic residues Glu121 and Glu141 are important for the antifungal effect of the cho product Trichormus fertilissimus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 More the enzyme belongs to the glycoside hydrolase 3, GH3-like family of Anabaena variabilis ATCC 29413 Trichormus fertilissimus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 30
-
 assay at Trichormus fertilissimus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 7
-
 assay at Trichormus fertilissimus

Expression

EC Number Organism Comment Expression
3.2.1.132 Trichormus fertilissimus the enzyme's chitosanase and antifungal activities are induced significantly under of growth-limiting conditions of 8 of light and 16 h of darkness up