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Literature summary extracted from

  • Wang, B.Z.; Guo, P.; Hang, B.J.; Li, L.; He, J.; Li, S.P.
    Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from Sphingobium sp. strain JZ-1 and characterization of the gene product (2009), Appl. Environ. Microbiol., 75, 5496-5500.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.88 expression in Escherichia coli BL21 Sphingobium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.88 Ag+ 0.5 mM, strong inhibition Sphingobium sp.
3.1.1.88 Cu2+ 0.5 mM, strong inhibition Sphingobium sp.
3.1.1.88 diethylpyrocarbonate 0.5 mM, strong inhibition Sphingobium sp.
3.1.1.88 Hg2+ 0.5 mM, strong inhibition Sphingobium sp.
3.1.1.88 iodoacetamide 0.5 mM, strong inhibition Sphingobium sp.
3.1.1.88 additional information 10 mM EDTA or 10 mM 1,10-phenanthroline have little effect on the enzyme activity (less than 10% inhibition) Sphingobium sp.
3.1.1.88 Ni2+ 0.5 mM, strong inhibition Sphingobium sp.
3.1.1.88 p-chloromercuribenzoate 0.5 mM, strong inhibition Sphingobium sp.
3.1.1.88 PMSF 0.5 mM, strong inhibition Sphingobium sp.
3.1.1.88 SDS 10 mM, strong inhibition Sphingobium sp.
3.1.1.88 Triton X-100 10 mM, 20-30% inhibition Sphingobium sp.
3.1.1.88 Tween 80 10 mM, strong inhibition Sphingobium sp.
3.1.1.88 Zn2+ 0.5 mM, strong inhibition Sphingobium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.88 0.000062
-
 trans-permethrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.000065
-
 cis-permethrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.000106
-
 (alphaR)-fenpropathrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.000106
-
 (alphaS)-fenpropathrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.000108
-
 cis-cypermethrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.00011
-
 trans-cypermethrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.000348
-
 cyhalothrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.000585
-
 (alphaS,2R)-fenvalerate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.000585
-
 (alphaS,2S)-fenvalerate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.000585
-
 (alphaR,2R)-fenvalerate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.000585
-
 (alphaR,2S)-fenvalerate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.000788
-
 deltamethrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.00159
-
 bifenthrin pH 7.5, 37°C Sphingobium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.88 30835
-
 1 * 30835, calculated from sequence Sphingobium sp.
3.1.1.88 31000
-
 gel filtration Sphingobium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.88 additional information Sphingobium sp. the enzyme is involved in degradation of pyrethroids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.88 Sphingobium sp. C0LA90 strain JZ-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.88
-
 Sphingobium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.88 0.84
-
 substrate: bifenthrin, pH 7.5, 37°C Sphingobium sp.
3.1.1.88 1.52
-
 substrate: deltamethrin, pH 7.5, 37°C Sphingobium sp.
3.1.1.88 1.75
-
 substrate: fenvalerate, pH 7.5, 37°C Sphingobium sp.
3.1.1.88 2.46
-
 substrate: cyhalothrin, pH 7.5, 37°C Sphingobium sp.
3.1.1.88 4.86
-
 substrate: trans-cypermethrin, pH 7.5, 37°C Sphingobium sp.
3.1.1.88 4.95
-
 substrate: cis-cypermethrin, pH 7.5, 37°C Sphingobium sp.
3.1.1.88 5.02
-
 substrate: fenpropathrin, pH 7.5, 37°C Sphingobium sp.
3.1.1.88 5.76
-
 substrate: cis-permethrin, pH 7.5, 37°C Sphingobium sp.
3.1.1.88 5.82
-
 substrate: trans-permethrin, pH 7.5, 37°C Sphingobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.88 (alphaR)-fenpropathrin + H2O both stereoisomers of fenpropathrin: (alphaR)- and (alphaS)-fenpropathrin are hydrolyzed at an appropriately equal rate Sphingobium sp. 3-phenoxybenzaldehyde + 2,2,3,3-tetramethylcyclopropanecarboxylate + cyanide
-
 ?
3.1.1.88 (alphaR,2R)-fenvalerate + H2O the four stereoisomers of fenvalerate: (alphaS,2R)-fenvalerate, (alphaR,2S)-fenvalerate, (alphaS,2S)-fenvalerate and (alphaR,2R)-fenvalerate are hydrolyzed at an appropriately equal rate Sphingobium sp. 3-phenoxybenzaldehyde + (2R)-2-(4-chlorophenyl)-3-methylbutanoate + cyanide
-
 ?
3.1.1.88 (alphaR,2S)-fenvalerate + H2O the four stereoisomers of fenvalerate: (alphaS,2R)-fenvalerate, (alphaR,2S)-fenvalerate, (alphaS,2S)-fenvalerate and (alphaR,2R)-fenvalerate are hydrolyzed at an appropriately equal rate Sphingobium sp. 3-phenoxybenzaldehyde + (2S)-2-(4-chlorophenyl)-3-methylbutanoate + cyanide
-
 ?
3.1.1.88 (alphaS)-fenpropathrin + H2O both stereoisomers of fenpropathrin: (alphaR)- and (alphaS)-fenpropathrin are hydrolyzed at an appropriately equal rate Sphingobium sp. 3-phenoxybenzaldehyde + 2,2,3,3-tetramethylcyclopropanecarboxylate + cyanide
-
 ?
3.1.1.88 (alphaS,2R)-fenvalerate + H2O the four stereoisomers of fenvalerate: (alphaS,2R)-fenvalerate, (alphaR,2S)-fenvalerate, (alphaS,2S)-fenvalerate and (alphaR,2R)-fenvalerate are hydrolyzed at an appropriately equal rate Sphingobium sp. 3-phenoxybenzaldehyde + (2R)-2-(4-chlorophenyl)-3-methylbutanoate + cyanide
-
 ?
3.1.1.88 (alphaS,2S)-fenvalerate + H2O the four stereoisomers of fenvalerate: (alphaS,2R)-fenvalerate, (alphaR,2S)-fenvalerate, (alphaS,2S)-fenvalerate and (alphaR,2R)-fenvalerate are hydrolyzed at an appropriately equal rate Sphingobium sp. 3-phenoxybenzaldehyde + (2S)-2-(4-chlorophenyl)-3-methylbutanoate + cyanide
-
 ?
3.1.1.88 bifenthrin + H2O
-
 Sphingobium sp. (2-methylbiphenyl-3-yl)methanol + 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylate
-
 ?
3.1.1.88 cis-cypermethrin + H2O
-
 Sphingobium sp. 3-phenoxybenzaldehyde + (1S,3S)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
 ?
3.1.1.88 cis-permethrin + H2O
-
 Sphingobium sp. (3-phenoxyphenyl)methanol + (1S,3S)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate
-
 ?
3.1.1.88 cyhalothrin + H2O
-
 Sphingobium sp. 3-phenoxybenzaldehyde + 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylate + cyanide
-
 ?
3.1.1.88 deltamethrin + H2O
-
 Sphingobium sp. 3-phenoxybenzaldehyde + (1R,3R)-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
 ?
3.1.1.88 additional information the enzyme is involved in degradation of pyrethroids Sphingobium sp. ?
-
 ?
3.1.1.88 trans-cypermethrin + H2O
-
 Sphingobium sp. 3-phenoxybenzaldehyde + (1S,3R)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
 ?
3.1.1.88 trans-permethrin + H2O
-
 Sphingobium sp. (3-phenoxyphenyl)methanol + (1S,3R)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.88 monomer 1 * 31000, SDS-PAGE Sphingobium sp.
3.1.1.88 monomer 1 * 30835, calculated from sequence Sphingobium sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.1.88 pyrethroid-hydrolyzing carboxylesterase
-
 Sphingobium sp.
3.1.1.88 pytH
-
 Sphingobium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.88 37
-
 assay at Sphingobium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.88 50
-
 1 h, stable up to Sphingobium sp.
3.1.1.88 60
-
 1 h, 45% loss of activity Sphingobium sp.
3.1.1.88 70
-
 1 h, complete inactivation Sphingobium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.88 0.44
-
 bifenthrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.79
-
 deltamethrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.91
-
 (alphaS,2R)-fenvalerate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.91
-
 (alphaS,2S)-fenvalerate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.91
-
 (alphaR,2R)-fenvalerate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 0.91
-
 (alphaR,2S)-fenvalerate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 1.28
-
 cyhalothrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 2.53
-
 trans-cypermethrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 2.57
-
 cis-cypermethrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 2.61
-
 (alphaR)-fenpropathrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 2.61
-
 (alphaS)-fenpropathrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 3
-
 cis-permethrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 3.03
-
 trans-permethrin pH 7.5, 37°C Sphingobium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.88 7.5
-
-
 Sphingobium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.88 5.5 9 4 h, enzyme retains more than 80% of its activity in this range Sphingobium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
3.1.1.88 additional information no cofactors were required for enzyme activity Sphingobium sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.88 Sphingobium sp. isoelectric focusing, pH-range: 3.5-10.0
-
 4.85

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.88 150
-
 4-nitrophenyl hexanoate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 270
-
 bifenthrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 670
-
 4-nitrophenyl butanoate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 1000
-
 deltamethrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 1370
-
 4-nitrophenyl acetate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 1560
-
 (alphaS,2R)-fenvalerate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 1560
-
 (alphaS,2S)-fenvalerate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 1560
-
 (alphaR,2R)-fenvalerate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 1560
-
 (alphaR,2S)-fenvalerate pH 7.5, 37°C Sphingobium sp.
3.1.1.88 3670
-
 cyhalothrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 23010
-
 trans-cypermethrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 23870
-
 cis-cypermethrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 24670
-
 (alphaR)-fenpropathrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 24670
-
 (alphaS)-fenpropathrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 46150
-
 cis-permethrin pH 7.5, 37°C Sphingobium sp.
3.1.1.88 48900
-
 trans-permethrin pH 7.5, 37°C Sphingobium sp.