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Literature summary extracted from

  • Kui, H.; Luo, H.; Shi, P.; Bai, Y.; Yuan, T.; Wang, Y.; Yang, P.; Dong, S.; Yao, B.
    Gene cloning, expression, and Characterization of a thermostable xylanase from Nesterenkonia xinjiangensis CCTCC AA001025 (2009), Appl. Biochem. Biotechnol., 162, 953-965.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 2-mercaptoethanol activates 1.9fold at 10 mM Nesterenkonia xinjiangensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 gene xyn11NX, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Nesterenkonia xinjiangensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ag+ complete inhibition at 1 mM Nesterenkonia xinjiangensis
3.2.1.8 Co2+
-
 Nesterenkonia xinjiangensis
3.2.1.8 Cu2+
-
 Nesterenkonia xinjiangensis
3.2.1.8 Hg2+ complete inhibition at 1 mM Nesterenkonia xinjiangensis
3.2.1.8 Pb2+
-
 Nesterenkonia xinjiangensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information kinetics Nesterenkonia xinjiangensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Ca2+ activates Nesterenkonia xinjiangensis
3.2.1.8 Cr3+ activates 1.3fold at 10 mM Nesterenkonia xinjiangensis
3.2.1.8 Fe2+ activates Nesterenkonia xinjiangensis
3.2.1.8 K+ activates Nesterenkonia xinjiangensis
3.2.1.8 Li+ activates Nesterenkonia xinjiangensis
3.2.1.8 Mg2+ activates Nesterenkonia xinjiangensis
3.2.1.8 Mn2+ activates Nesterenkonia xinjiangensis
3.2.1.8 Na+ activates Nesterenkonia xinjiangensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 26000
-
 x * 26000, recombinantenzyme, SDS-PAGE Nesterenkonia xinjiangensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O Nesterenkonia xinjiangensis
-
 ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O Nesterenkonia xinjiangensis CCTCC AA001025
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Nesterenkonia xinjiangensis D1KJJ7 gene xyn11NX
-
3.2.1.8 Nesterenkonia xinjiangensis CCTCC AA001025 D1KJJ7 gene xyn11NX
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homnogeneity Nesterenkonia xinjiangensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O
-
 Nesterenkonia xinjiangensis ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O
-
 Nesterenkonia xinjiangensis CCTCC AA001025 ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O from birchwood or oat spelt Nesterenkonia xinjiangensis xylobiose + xylotriose + ? most predominant products. Hydrolysis products of oat spelt xylan is 2.3 mM xylobiose and 0.652 mM xylotriose, and of birchwood xylan 4.95 mM xylobiose and 1.57 mM xylotriose ?
3.2.1.8 beta-1,4-xylan + H2O from birchwood or oat spelt Nesterenkonia xinjiangensis CCTCC AA001025 xylobiose + xylotriose + ? most predominant products. Hydrolysis products of oat spelt xylan is 2.3 mM xylobiose and 0.652 mM xylotriose, and of birchwood xylan 4.95 mM xylobiose and 1.57 mM xylotriose ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 26000, recombinantenzyme, SDS-PAGE Nesterenkonia xinjiangensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 More the enzyme belongs to glycosyl hydrolase family 11, GH11 Nesterenkonia xinjiangensis
3.2.1.8 Xyn11NX
-
 Nesterenkonia xinjiangensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 55
-
 recombinant enzyme Nesterenkonia xinjiangensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 20 70 activity range, profile, overview Nesterenkonia xinjiangensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 45 60 purified enzyme, pH 7.0, 60 min, 80% activity remaining Nesterenkonia xinjiangensis
3.2.1.8 70
-
 purified enzyme, pH 7.0, 60 min, 55% activity remaining Nesterenkonia xinjiangensis
3.2.1.8 80
-
 purified enzyme, pH 7.0, 60 min, 10% activity remaining Nesterenkonia xinjiangensis
3.2.1.8 90
-
 purified enzyme, pH 7.0, 60 min, 5% activity remaining Nesterenkonia xinjiangensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 6.37
-
 beta-1,4-xylan pH 7.0, 55°C, recombinant enzyme, birchwood xylan Nesterenkonia xinjiangensis
3.2.1.8 18.12
-
 beta-1,4-xylan pH 7.0, 55°C, recombinant enzyme, oat spelt xylan Nesterenkonia xinjiangensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 7
-
 recombinant enzyme Nesterenkonia xinjiangensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 2 12 60% of maximal activity at pH 6.0 and pH 8.0, pH profile, recombinant enzyme, overview Nesterenkonia xinjiangensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 5 11 purified recombinant enzyme, the enzyme retains more than 80% of initial activity after 1 h at pH 5.0-11.0 at 37 °C for 1 h Nesterenkonia xinjiangensis