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Literature summary extracted from

  • Sung, J.H.; Ahn, S.J.; Kim, N.Y.; Jeong, S.K.; Kim, J.K.; Chung, J.K.; Lee, H.H.
    Purification, molecular cloning, and biochemical characterization of subtilisin JB1 from a newly isolated Bacillus subtilis JB1 (2010), Appl. Biochem. Biotechnol., 162, 900-911.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.62 industry subtilisin JB1 may serve as a potential source material for use in industrial applications of proteolytic enzymes and microorganisms for fishery waste degradation and fish by-product processing Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.62 prosubtilisin JB1 cloned into the GST fusion protein expression vector pGEX-4T-1 and transformed into Escherichia coli DH5alphaMCR Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.62 1,10-phenanthroline at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 40% Bacillus subtilis
3.4.21.62 antipain at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 75% Bacillus subtilis
3.4.21.62 Brij 35 at 37°C and pH of 7.5, 0.01% reduces prosubtilisin JB1 relative activity to 30% and 0.05% reduces prosubtilisin JB1 relative activity to 70% Bacillus subtilis
3.4.21.62 Ca2+ at 37°C and pH of 7.5, 1 mM reduces prosubtilisin JB1 relative activity to 57% and 5 mM reduces prosubtilisin JB1 relative activity to 45% Bacillus subtilis
3.4.21.62 chymostatin at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 95% Bacillus subtilis
3.4.21.62 Co2+ at 37°C and pH of 7.5, 1 mM reduces prosubtilisin JB1 relative activity to 14% and 5 mM reduces prosubtilisin JB1 relative activity to 24% Bacillus subtilis
3.4.21.62 Cu2+ at 37°C and pH of 7.5, with 1 mM results in an almost complete reduction of prosubtilisin JB1 activity, 5 mM reduces prosubtilisin JB1 relative activity to 19% Bacillus subtilis
3.4.21.62 E-64 at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 31% Bacillus subtilis
3.4.21.62 E-64c at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 25% Bacillus subtilis
3.4.21.62 EDTA at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 52% Bacillus subtilis
3.4.21.62 EGTA at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 34% Bacillus subtilis
3.4.21.62 Hg+ at 37°C and pH of 7.5, 1 mM reduces prosubtilisin JB1 relative activity to 33% and 5 mM reduces prosubtilisin JB1 relative activity to 22% Bacillus subtilis
3.4.21.62 K+ at 37°C and pH of 7.5, with 1 mM results in an almost complete reduction of prosubtilisin JB1 activity, 5 mM reduces prosubtilisin JB1 relative activity to 53% Bacillus subtilis
3.4.21.62 Mg2+ at 37°C and pH of 7.5, 1 mM reduces prosubtilisin JB1 relative activity to 79% and 5 mM reduces prosubtilisin JB1 relative activity to 48% Bacillus subtilis
3.4.21.62 additional information at 37°C and pH of 7.5, 0.1 mM leupeptin does not inhibit prosubtilisin JB1 Bacillus subtilis
3.4.21.62 N-ethylmaleimide at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 15% Bacillus subtilis
3.4.21.62 pepstatin A at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 48% Bacillus subtilis
3.4.21.62 phenylmethylsulfonyl fluoride at 37°C and pH of 7.5, 0.1 mM inhibits prosubtilisin JB1 by 79% Bacillus subtilis
3.4.21.62 Sodium dodecyl sulfate at 37°C and pH of 7.5, 0.01% reduces prosubtilisin JB1 relative activity to 52% and 0.05% reduces prosubtilisin JB1 relative activity to 29% Bacillus subtilis
3.4.21.62 Triton X-100 at 37°C and pH of 7.5, 0.01% reduces prosubtilisin JB1 relative activity to 92% and 0.05% reduces prosubtilisin JB1 relative activity to 69% Bacillus subtilis
3.4.21.62 Tween 20 at 37°C and pH of 7.5, 0.01% reduces prosubtilisin JB1 relative activity to 53% and 0.05% reduces prosubtilisin JB1 relative activity to 70% Bacillus subtilis
3.4.21.62 Zn2+ at 37°C and pH of 7.5, 1 mM reduces prosubtilisin JB1 relative activity to 30%, with 5 mM results in an almost complete reduction of prosubtilisin JB1 activity Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.62 62000
-
 purified prosubtilisin JB1, Western blotting analysis Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Bacillus subtilis B6VFQ8 JB1
-
3.4.21.62 Bacillus subtilis JB1 B6VFQ8 JB1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.62 prosubtilisin purified by ammonium sulfate precipitation and gel filtration Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 Ala-Ala-Phe 7-amido-4-methylcoumarin + H2O
-
 Bacillus subtilis ?
-
 ?
3.4.21.62 Ala-Ala-Phe 7-amido-4-methylcoumarin + H2O
-
 Bacillus subtilis JB1 ?
-
 ?
3.4.21.62 azocasein + H2O
-
 Bacillus subtilis ?
-
 ?
3.4.21.62 azocasein + H2O
-
 Bacillus subtilis JB1 ?
-
 ?
3.4.21.62 Bovine serum albumin + H2O
-
 Bacillus subtilis ?
-
 ?
3.4.21.62 Bovine serum albumin + H2O
-
 Bacillus subtilis JB1 ?
-
 ?
3.4.21.62 Fibrinogen + H2O
-
 Bacillus subtilis ?
-
 ?
3.4.21.62 Fibrinogen + H2O
-
 Bacillus subtilis JB1 ?
-
 ?
3.4.21.62 Gelatin + H2O
-
 Bacillus subtilis ?
-
 ?
3.4.21.62 Gelatin + H2O
-
 Bacillus subtilis JB1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.62 subtilisin JB1
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.62 7.5
-
 recombinant prosubtilisin JB1, at 37°C Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.62 4 8 recombinant prosubtilisin JB1, at 37°C Bacillus subtilis