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Literature summary extracted from

  • Shanmughapriya, S.; Kiran, G.S.; Selvin, J.; Thomas, T.A.; Rani, C.
    Optimization, purification, and characterization of extracellular mesophilic alkaline cellulase from sponge-associated Marinobacter sp. MSI032 (2009), Appl. Biochem. Biotechnol., 162, 625-640.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 DNA and amino acid sequence determination and analysis, phylogenetic tree and analysis Marinobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Ca2+
-
 Marinobacter sp.
3.2.1.4 Fe2+
-
 Marinobacter sp.
3.2.1.4 Mn2+
-
 Marinobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.4 extracellular
-
 Marinobacter sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Mg2+ slightly activating Marinobacter sp.
3.2.1.4 additional information enzyme activity is not affected by Hg2+ Marinobacter sp.
3.2.1.4 NaCl 100% activation at 2.5 M Marinobacter sp.
3.2.1.4 Zn2+ highly activating Marinobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 68000
-
 x * 68000, SDS-PAGE Marinobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.4 cellulose + H2O Marinobacter sp.
-
 ?
-
 ?
3.2.1.4 cellulose + H2O Marinobacter sp. MSI032
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Marinobacter sp.
-
 isolated from the marine sponge Dendrilla nigra
-
3.2.1.4 Marinobacter sp. MSI032
-
 isolated from the marine sponge Dendrilla nigra
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 native extracellular enzyme 12.5fold from culture medium by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography Marinobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.4 additional information the culture medium containing 1% maltose as carbon source and 1% peptone and casein as nitrogen source, and with presence of 2% NaCl and 10 mM Zn2+, supports maximal enzyme production at 27°C and at a pH of 9.0 Marinobacter sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 2549
-
 purified native enzyme Marinobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 cellulose + H2O
-
 Marinobacter sp. ?
-
 ?
3.2.1.4 cellulose + H2O
-
 Marinobacter sp. MSI032 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 68000, SDS-PAGE Marinobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Alkaline cellulase
-
 Marinobacter sp.
3.2.1.4 CMCase
-
 Marinobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 27 35
-
 Marinobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 27 30 purified enzyme, 60 min, completely stable Marinobacter sp.
3.2.1.4 37
-
 purified enzyme, loss of 60% activity after 60 min Marinobacter sp.
3.2.1.4 40
-
 purified enzyme, inactivation after 60 min Marinobacter sp.
3.2.1.4 50
-
 purified enzyme, inactivation after 40 min Marinobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 9
-
-
 Marinobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 6 12 the enzyme shows a broad pH range with 50% of maximal activity at pH 7.0 and maximal activity at pH 9.0 Marinobacter sp.