Literature summary extracted from
Liu, W.; Shi, P.; Chen, Q.; Yang, P.; Wang, G.; Wang, Y.; Luo, H.; Yao, B.
Gene cloning, overexpression, and characterization of a xylanase from Penicillium sp. CGMCC 1669 (2010), Appl. Biochem. Biotechnol., 162, 1-12.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.2.1.8 |
2-mercaptoethanol |
activates by 35% at 10 mM |
Penicillium sp. CGMCC 1669 |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.8 |
gene xyn11F63, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, overexpression in Pichia pastoris, the recombinant enzyme is secreted to the culture medium |
Penicillium sp. CGMCC 1669 |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.2.1.8 |
Ag+ |
90% inhibition at 5 mM |
Penicillium sp. CGMCC 1669 |
|
3.2.1.8 |
Co2+ |
24% inhibition at 10 mM |
Penicillium sp. CGMCC 1669 |
|
3.2.1.8 |
Cr3+ |
18% inhibition at 10 mM |
Penicillium sp. CGMCC 1669 |
|
3.2.1.8 |
Cu2+ |
40% inhibition at 5-10 mM |
Penicillium sp. CGMCC 1669 |
|
3.2.1.8 |
Fe3+ |
40% inhibition at 10 mM |
Penicillium sp. CGMCC 1669 |
|
3.2.1.8 |
Hg2+ |
complete inhibition at 5 mM |
Penicillium sp. CGMCC 1669 |
|
3.2.1.8 |
Mn2+ |
10% inhibition at 1 mM |
Penicillium sp. CGMCC 1669 |
|
3.2.1.8 |
additional information |
no or poor inhibition by Na+, Li+, K+, Mg2+, and Ca2+ |
Penicillium sp. CGMCC 1669 |
|
3.2.1.8 |
Ni2+ |
10% inhibition at 5-10 mM |
Penicillium sp. CGMCC 1669 |
|
3.2.1.8 |
Pb2+ |
42% inhibition at 10 mM |
Penicillium sp. CGMCC 1669 |
|
3.2.1.8 |
SDS |
54% inhibition at 1 mM, 92% at 10 mM |
Penicillium sp. CGMCC 1669 |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.2.1.8 |
additional information |
- |
additional information |
kinetics |
Penicillium sp. CGMCC 1669 |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.2.1.8 |
21500 |
- |
x * 21500, about, sequence calculation |
Penicillium sp. CGMCC 1669 |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.2.1.8 |
beta-1,4-xylan + H2O |
Penicillium sp. CGMCC 1669 |
- |
? |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.8 |
Penicillium sp. CGMCC 1669 |
D1GFE6 |
gene xyn11F63; gene xyn11F63 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.8 |
recombinantz enzyme 2.33fold from Pichia pastoris to homogeneity from culture medium |
Penicillium sp. CGMCC 1669 |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.2.1.8 |
7988 |
- |
purified recombinant enzyme |
Penicillium sp. CGMCC 1669 |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.8 |
beta-1,4-xylan + H2O |
- |
Penicillium sp. CGMCC 1669 |
? |
- |
? |
|
3.2.1.8 |
beta-1,4-xylan + H2O |
from birchwood or oat spelt |
Penicillium sp. CGMCC 1669 |
D-xylose + 1,4-beta-D-xylobiose + 1,4-beta-D-xylotriose + 1,4-beta-D-xylotetraose |
from oat spelt xylan results 7.46% xylose, 28.36% xylobiose, 25.37% xylotriose, and 38.81% xylotetraose |
? |
|
3.2.1.8 |
additional information |
no activity towards carboxymethylcellulose-sodium, lichenan, or barley beta-glucan |
Penicillium sp. CGMCC 1669 |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.8 |
? |
x * 21500, about, sequence calculation |
Penicillium sp. CGMCC 1669 |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.8 |
XYN11F63 |
- |
Penicillium sp. CGMCC 1669 |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.2.1.8 |
40 |
- |
recombinant enzyme |
Penicillium sp. CGMCC 1669 |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
3.2.1.8 |
5416 |
- |
beta-1,4-xylan |
recombinant enzyme, pH 4.5, 37°C |
Penicillium sp. CGMCC 1669 |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.2.1.8 |
4.5 |
- |
recombinant enzyme |
Penicillium sp. CGMCC 1669 |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
3.2.1.8 |
4.5 |
9 |
purified recombinant enzyme, stable |
Penicillium sp. CGMCC 1669 |