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Literature summary extracted from

  • Jordan, D.B.; Braker, J.D.
    beta-D-Xylosidase from Selenomonas ruminantium: role of glutamate 186 in catalysis revealed by site-directed mutagenesis, alternate substrates, and active-site inhibitor (2010), Appl. Biochem. Biotechnol., 161, 395-410.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.37 E186A The E186A mutation abolishes the pKa assigned to the catalytic acid E186, mutant enzyme binds only the neutral aminoalcohol triethanolamine, whereas wild-type enzyme binds only the cationic aminoalcohol. At pH 7.0 and 25°C, the ratio of kcat values for substrates 4-nitrophenyl-beta-D-xylopyranoside and 4-nitrophenyl-alpha-L-arabinofuranoside of E186A is 100fold that of wild-type enzyme Selenomonas ruminantium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 triethanolamine
-
 Selenomonas ruminantium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.133
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 8.0, 25°C Selenomonas ruminantium
3.2.1.37 0.152
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 0.177
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 6.0, 25°C Selenomonas ruminantium
3.2.1.37 0.33
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 8.0, 25°C Selenomonas ruminantium
3.2.1.37 0.39
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 0.599
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 6.0, 25°C Selenomonas ruminantium
3.2.1.37 0.715
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 6.0, 25°C Selenomonas ruminantium
3.2.1.37 1.08
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 2.57
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 8.0, 25°C Selenomonas ruminantium
3.2.1.37 2.82
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 6.0, 25°C Selenomonas ruminantium
3.2.1.37 3.27
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 4.09
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 8.0, 25°C Selenomonas ruminantium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Selenomonas ruminantium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Selenomonas ruminantium 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Selenomonas ruminantium 4-nitrophenol + beta-D-xylopyranose
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 0.0000036
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 0.0000044
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 8.0, 25°C Selenomonas ruminantium
3.2.1.37 0.0000045
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 6.0, 25°C Selenomonas ruminantium
3.2.1.37 0.0014
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 6.0, 25°C Selenomonas ruminantium
3.2.1.37 0.0016
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 0.0016
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 8.0, 25°C Selenomonas ruminantium
3.2.1.37 2.36
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 8.0, 25°C Selenomonas ruminantium
3.2.1.37 3.05
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 8.0, 25°C Selenomonas ruminantium
3.2.1.37 3.13
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 6.0, 25°C Selenomonas ruminantium
3.2.1.37 3.28
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 14.4
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 29.3
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 6.0, 25°C Selenomonas ruminantium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.37 0.088
-
 triethanolamine wild-type, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 8.0, 25°C Selenomonas ruminantium
3.2.1.37 0.111
-
 triethanolamine wild-type, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 0.121
-
 triethanolamine wild-type, substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 0.54
-
 triethanolamine wild-type, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 6.0, 25°C Selenomonas ruminantium
3.2.1.37 33.2
-
 triethanolamine mutant E186A, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 8.0, 25°C Selenomonas ruminantium
3.2.1.37 47
-
 triethanolamine mutant E186A, substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 8.0, 25°C Selenomonas ruminantium
3.2.1.37 145
-
 triethanolamine mutant E186A, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 418
-
 triethanolamine mutant E186A, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 6.0, 25°C Selenomonas ruminantium
3.2.1.37 815
-
 triethanolamine mutant E186A, substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 7.0, 25°C Selenomonas ruminantium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.37 0.000024
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 0.000025
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 6.0, 25°C Selenomonas ruminantium
3.2.1.37 0.000033
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 8.0, 25°C Selenomonas ruminantium
3.2.1.37 0.0005
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 0.00051
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 6.0, 25°C Selenomonas ruminantium
3.2.1.37 0.00062
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 8.0, 25°C Selenomonas ruminantium
3.2.1.37 0.746
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 8.0, 25°C Selenomonas ruminantium
3.2.1.37 3.03
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 4.38
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 6.0, 25°C Selenomonas ruminantium
3.2.1.37 7.13
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 8.0, 25°C Selenomonas ruminantium
3.2.1.37 36.4
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 7.0, 25°C Selenomonas ruminantium
3.2.1.37 48.9
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 6.0, 25°C Selenomonas ruminantium