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Literature summary extracted from

  • Qiao, J.; Dong, B.; Li, Y.; Zhang, B.; Cao, Y.
    Cloning of a beta-1,3-1,4-glucanase gene from Bacillus subtilis MA139 and its functional expression in Escherichia coli (2009), Appl. Biochem. Biotechnol., 152, 334-342.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.6 arginine 8% activation at 10 mM Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.6 DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain Bl21 Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.6 Cu2+ 97% inhibition at 10 mM Bacillus subtilis
3.2.1.6 EDTA 28.5% inhibition at 10 mM Bacillus subtilis
3.2.1.6 Mn2+ 54% inhibition at 10 mM Bacillus subtilis
3.2.1.6 Na2MoO4 20% inhibition at 10 mM Bacillus subtilis
3.2.1.6 Zn2+ 7.6% inhibition at 10 mM Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.6 additional information
-
 additional information kinetics Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.6 Fe2+ 9% activation at 10 mM Bacillus subtilis
3.2.1.6 additional information the enzyme activity is not affected by Mg2+, Ca2+, KI, and sucrose at 10 mM Bacillus subtilis
3.2.1.6 Na2SeO3 9% activation at 10 mM Bacillus subtilis
3.2.1.6 NaCl 12.5% activation at 10 mM Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.6 24440
-
 x * 24440, sequence calculation, x * 25500, recombinant His-tagged enzyme, SDS-PAGE Bacillus subtilis
3.2.1.6 25500
-
 x * 24440, sequence calculation, x * 25500, recombinant His-tagged enzyme, SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Bacillus subtilis A8CGP1 isolated from pig intestine
-
3.2.1.6 Bacillus subtilis MA139 A8CGP1 isolated from pig intestine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.6 recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.6 379.1
-
 purified recombinant His-tagged enzyme, substrate lichenan Bacillus subtilis
3.2.1.6 728.8
-
 purified recombinant His-tagged enzyme, substrate beta-glucan from Hordeum vulgare Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 beta-1,3-1,4-glucan + H2O from Hordeum vulgare, preferred substrate Bacillus subtilis ?
-
 ?
3.2.1.6 beta-1,3-1,4-glucan + H2O from Hordeum vulgare, preferred substrate Bacillus subtilis MA139 ?
-
 ?
3.2.1.6 lichenan + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.6 lichenan + H2O
-
 Bacillus subtilis MA139 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.6 ? x * 24440, sequence calculation, x * 25500, recombinant His-tagged enzyme, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.6 beta-1,3-1,4-glucanase
-
 Bacillus subtilis
3.2.1.6 More the bacterial beta-1,3-1,4-glucanase belongs to the glycosyl hydrolase family 16, GH16, possessing the conserved EIDIEF motif Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.6 40
-
 recombinant enzyme Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.6 20 65 inactivation above 70°C, profile, overview Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.6 40
-
 purified recombinant His-tagged enzyme, 80% remaining activity after 2 h Bacillus subtilis
3.2.1.6 50
-
 purified recombinant His-tagged enzyme, 70% remaining activity after 2 h Bacillus subtilis
3.2.1.6 60
-
 purified recombinant His-tagged enzyme, 20% remaining activity after 30 min, below 10% after 60 min Bacillus subtilis
3.2.1.6 70
-
 purified recombinant His-tagged enzyme, inactivation within 10 min Bacillus subtilis
3.2.1.6 80
-
 purified recombinant His-tagged enzyme, inactivation within 5 min Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.6 1350
-
 lichenan pH 6.4, 40°C, substrate from Hordeum vulgare Bacillus subtilis
3.2.1.6 7207
-
 beta-1,3-1,4-glucan pH 6.4, 40°C, purified recombinant His-tagged enzyme, substrate from Hordeum vulgare Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.6 6.4
-
 recombinant enzyme Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.6 5 8 profile, overview Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.6 4 10 purified recombinant His-tagged enzyme, 40°C, 1 h, stable Bacillus subtilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.6 Bacillus subtilis sequence calculation
-
 5.55