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Literature summary extracted from

  • da Silva, T.M.; Michelin, M.; Damasio, A.R.; Maller, A.; Almeida, F.B.; Ruller, R.; Ward, R.J.; Rosa, J.C.; Jorge, J.A.; Terenzi, H.F.; Polizeli, M.d.e..L.
    Purification and biochemical characterization of a novel alpha-glucosidase from Aspergillus niveus (2009), Antonie van Leeuwenhoek, 96, 569-578.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 Ag2+ 10 mM Aspergillus niveus
3.2.1.20 beta-mercaptoethanol 10 mM Aspergillus niveus
3.2.1.20 Fe2+ 10 mM Aspergillus niveus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 additional information
-
 glycogen 0.04 mg/ml Aspergillus niveus
3.2.1.20 additional information
-
 starch 0.07 mg/ml Aspergillus niveus
3.2.1.20 additional information
-
 amylopectin 0.11 mg/ml Aspergillus niveus
3.2.1.20 additional information
-
 maltose 0.57 mg/ml Aspergillus niveus
3.2.1.20 0.55
-
 p-nitrophenyl alpha-D-glucopyranoside
-
 Aspergillus niveus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 extracellular
-
 Aspergillus niveus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.20 Ag2+ inhibitor, 10 mM Aspergillus niveus
3.2.1.20 Fe2+ inhibitor, 10 mM Aspergillus niveus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 52000
-
 Bio-Sil-Sec-400 gel filtration column Aspergillus niveus
3.2.1.20 56000
-
 SDS-PAGE Aspergillus niveus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Aspergillus niveus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 DEAE-Fractogel ion-exchange chromatography and Sephacryl S-200 gel filtration Aspergillus niveus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 additional information
-
 0.2 micromol/min/ml, substrate trehalose Aspergillus niveus
3.2.1.20 additional information
-
 0.3 micromol/min/ml, substrate isomaltose Aspergillus niveus
3.2.1.20 additional information
-
 0.4 micromol/min/ml, substrate beta-cyclodextrin Aspergillus niveus
3.2.1.20 additional information
-
 1.2 micromol/min/ml, substrate p-nitrophenyl alpha-D-glucopyranoside Aspergillus niveus
3.2.1.20 additional information
-
 2.7 micromol/min/ml, substrate maltose Aspergillus niveus
3.2.1.20 additional information
-
 4.2 micromol/min/ml, substrate amylopectin Aspergillus niveus
3.2.1.20 additional information
-
 4.2 micromol/min/ml, substrate maltooligosaccharide Aspergillus niveus
3.2.1.20 additional information
-
 4.2 micromol/min/ml, substrate maltotriose Aspergillus niveus
3.2.1.20 additional information
-
 4.3 micromol/min/ml, substrate maltotetraose Aspergillus niveus
3.2.1.20 additional information
-
 4.3 micromol/min/ml, substrate starch Aspergillus niveus
3.2.1.20 additional information
-
 4.4 micromol/min/ml, substrate amylose Aspergillus niveus
3.2.1.20 additional information
-
 4.5 micromol/min/ml, substrate glycogen Aspergillus niveus
3.2.1.20 additional information
-
 4.5 micromol/min/ml, substrate maltopentaose Aspergillus niveus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O assay at 65°C, pH 5.0, reaction stopped with Na2CO3 Aspergillus niveus 4-nitrophenol + D-glucose
-
 ?
3.2.1.20 amylopectin + H2O assay at 65°C, pH 5.0, reaction stopped with Na2CO3 Aspergillus niveus ?
-
 ?
3.2.1.20 glycogen + H2O assay at 65°C, pH 5.0, reaction stopped with Na2CO3 Aspergillus niveus ?
-
 ?
3.2.1.20 maltose + H2O assay at 65°C, pH 5.0, reaction stopped with Na2CO3 Aspergillus niveus ?
-
 ?
3.2.1.20 starch + H2O assay at 65°C, pH 5.0, reaction stopped with Na2CO3 Aspergillus niveus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 alpha-glucosidase
-
 Aspergillus niveus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 65
-
-
 Aspergillus niveus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 60 65 half-life at 60°C 104 min, half-life at 65°C 78 min Aspergillus niveus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 0.53
-
 p-nitrophenyl alpha-D-glucopyranoside
-
 Aspergillus niveus
3.2.1.20 13.66
-
 maltose
-
 Aspergillus niveus
3.2.1.20 24.5
-
 glycogen
-
 Aspergillus niveus
3.2.1.20 24.62
-
 starch
-
 Aspergillus niveus
3.2.1.20 25.48
-
 amylopectin
-
 Aspergillus niveus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 6
-
-
 Aspergillus niveus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 4 8.5 acid-alkaline tolerant, remains stable after 2 h incubation Aspergillus niveus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.20 additional information
-
 p-nitrophenyl alpha-D-glucopyranoside 0.96 1/s*mg*ml Aspergillus niveus
3.2.1.20 additional information
-
 maltose 23.96 1/s*mg*ml Aspergillus niveus
3.2.1.20 additional information
-
 amylopectin 231.64 1/s*mg*ml Aspergillus niveus
3.2.1.20 additional information
-
 starch 351.71 1/s*mg*ml Aspergillus niveus
3.2.1.20 additional information
-
 glycogen 612.5 1/s*mg*ml Aspergillus niveus