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Literature summary extracted from

  • Winkler, M.; Domarkas, J.; Schweiger, L.; OHagan, D.
    Fluorinase-coupled base swaps: Synthesis of 18F-5'-Deoxy-5'-fluorouridines (2008), Angew. Chem. Int. Ed. Engl., 47, 10141-10143.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.63 biotechnology production of radiolabelled nucleosides as tracers for cancer cell uptake studies via positron emission tomography Streptomyces cattleya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride Streptomyces cattleya 18F-tagged substrate with 3 L-amino acid oxidase to remove the byproduct L-methionine enhancing the production reaction, 37°C, 100 mM Tris-HCl buffer, pH 7.5, up to 4 hours, to produce 18F-tagged product, other enzymes can be added to produce several types of nucleosides 5'-deoxy-5'-fluoroadenosine + L-methionine
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Organism

EC Number Organism UniProt Comment Textmining
2.5.1.63 Streptomyces cattleya
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride 18F-tagged substrate with 3 L-amino acid oxidase to remove the byproduct L-methionine enhancing the production reaction, 37°C, 100 mM Tris-HCl buffer, pH 7.5, up to 4 hours, to produce 18F-tagged product, other enzymes can be added to produce several types of nucleosides Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.63 fluorinase enzyme
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 Streptomyces cattleya