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Literature summary extracted from

  • Evnouchidou, I.; Berardi, M.J.; Stratikos, E.
    A continuous fluorigenic assay for the measurement of the activity of endoplasmic reticulum aminopeptidase 1: competition kinetics as a tool for enzyme specificity investigation (2009), Anal. Biochem., 395, 33-40.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.22 expressed in insect cells driven by a baculoviral vector Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.22 amastatin about 50% inhibition at 0.1 mM Homo sapiens
3.4.11.22 leucinethiol about 80% inhibition at 0.1 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.22 0.075
-
 WRVYEKCALK at 37°C Homo sapiens
3.4.11.22 0.328
-
 WEVYEKCALK at 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.22 additional information Homo sapiens ERAP1 trims a large variety of long peptide sequences efficiently, and its activity drops dramatically for peptides shorter than eight or nine amino acids, the enzyme efficiently excises virtually any amino acid in the context of a larger peptide, ERAP1 has broad N-terminal specificity due to strong preferences for residues downstream in the peptide-substrate sequence ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.22 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.22 Ni-NTA column chromatography, MonoQ column chromatography, and Superdex S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.22 additional information ERAP1 trims a large variety of long peptide sequences efficiently, and its activity drops dramatically for peptides shorter than eight or nine amino acids, the enzyme efficiently excises virtually any amino acid in the context of a larger peptide, ERAP1 has broad N-terminal specificity due to strong preferences for residues downstream in the peptide-substrate sequence Homo sapiens ?
-
 ?
3.4.11.22 WEVYEKCALK + H2O
-
 Homo sapiens L-Trp + EVYEKCALK
-
 ?
3.4.11.22 WRVYEKCALK + H2O
-
 Homo sapiens L-Trp + RVYEKCALK
-
 ?
3.4.11.22 WRVYEKMALKC + H2O
-
 Homo sapiens L-Trp + RVYEKMALKC
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.22 endoplasmic reticulum aminopeptidase 1
-
 Homo sapiens
3.4.11.22 endoplasmic reticulum aminopeptidase associated with antigen presentation
-
 Homo sapiens
3.4.11.22 ERAAP
-
 Homo sapiens
3.4.11.22 ERAP1
-
 Homo sapiens