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Literature summary extracted from
- Spectrophotometric determination of phosphatidic acid via iron(III) complexation for assaying phospholipase D activity (2009), Anal. Biochem., 392, 169-173.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.4 | expression of isozyme PLD2alpha in Escherichia coli | Brassica oleracea |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | Ca2+ | stimulates | Brassica oleracea |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | phosphatidylcholine + H2O | Brassica oleracea | - |
choline + phosphatidic acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | Brassica oleracea | - |
white cabbage, isozyme PLD2alpha | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | leaf | - |
Brassica oleracea | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | additional information | spectrophotometric determination of phosphatidic acid via iron(III) complexation in presence of salicylate for simply assaying phospholipase D activity, method evaluation and optimization, overview | Brassica oleracea | ? | - |
? | |
| 3.1.4.4 | phosphatidylcholine + H2O | - |
Brassica oleracea | choline + phosphatidic acid | - |
? | |
| 3.1.4.4 | phosphatidylethanolamine + H2O | - |
Brassica oleracea | ethanolamine + phosphatidic acid | - |
? | |
| 3.1.4.4 | phosphatidylglycerol + H2O | - |
Brassica oleracea | glycerol + phosphatidic acid | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.4 | PLD | - |
Brassica oleracea |
| 3.1.4.4 | PLD2alpha | - |
Brassica oleracea |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 30 | - |
assay at | Brassica oleracea |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 5.6 | - |
assay at | Brassica oleracea |
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Release 2023.1 (January 2023)
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