Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Huang, H.; Tanaka, H.; Hammock, B.D.; Morisseau, C.
    Novel and highly sensitive fluorescent assay for leucine aminopeptidases (2009), Anal. Biochem., 391, 11-16.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 bestatin
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.065
-
 N-(6-methoxypyridin-3-yl)-(S)-2-amino-4-methylpentanamide pH 8.0, 31°C Sus scrofa
3.4.11.1 3
-
 L-leucine-4-nitroanilide pH 8.0, 31°C Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.1 microsome
-
 Sus scrofa
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 kidney
-
 Sus scrofa
-
3.4.11.1 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 0.044
-
 kidney enzyme, substrate is N-(6-methoxy-2-methylpyridin-3-yl)-(S)-2-amino-4-methylpentanamide Sus scrofa
3.4.11.1 5.1
-
 kidney enzyme, substrate is L-leucine-4-nitroanilide Sus scrofa
3.4.11.1 6.5
-
 kidney enzyme, substrate is N-(6-methoxypyridin-3-yl)-(S)-2-amino-4-methylpentanamide Sus scrofa
3.4.11.1 8.4
-
 kidney enzyme, substrate is (S)-2-amino-N-(6-(dimethylamino)pyridin-3-yl)-4-methylpentanamide Sus scrofa
3.4.11.1 9.6
-
 kidney enzyme, substrate is L-leucyl-4-methylcoumaryl 7-amide Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 (S)-2-amino-N-(6-(dimethylamino)pyridin-3-yl)-4-methylpentanamide + H2O
-
 Sus scrofa ?
-
 ?
3.4.11.1 L-leucine-4-methylcoumaryl-7-amide + H2O
-
 Sus scrofa L-leucine + 7-amino-4-methylcoumarine
-
 ?
3.4.11.1 L-leucine-4-nitroanilide + H2O
-
 Sus scrofa L-leucine + 4-nitroaniline
-
 ?
3.4.11.1 additional information substrate synthesis and assay method optimization, overview Sus scrofa ?
-
 ?
3.4.11.1 N-(6-methoxy-2-methylpyridin-3-yl)-(S)-2-amino-4-methylpentanamide + H2O
-
 Sus scrofa ?
-
 ?
3.4.11.1 N-(6-methoxypyridin-3-yl)-(S)-2-amino-4-methylpentanamide + H2O
-
 Sus scrofa ?
-
 ?
3.4.11.1 N-(tert-butoxycarbonyl)-L-leucine + H2O
-
 Sus scrofa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 LAP
-
 Sus scrofa
3.4.11.1 leucine aminopeptidase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 31
-
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8
-
-
 Sus scrofa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.11.1 0.007
-
 pH 8.0, 31°C, versus substrate L-leucine-4-nitroanilide Sus scrofa bestatin
3.4.11.1 0.025
-
 pH 8.0, 31°C, versus substrate N-(6-methoxypyridin-3-yl)-(S)-2-amino-4-methylpentanamide Sus scrofa bestatin