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Literature summary extracted from

  • Havriluk, T.; Lozy, F.; Siniossoglou, S.; Carman, G.
    Colorimetric determination of pure Mg2+-dependent phosphatidate phosphatase activity (2008), Anal. Biochem., 373, 392-394.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.3.4 drug development the PAP1 colorimetric assay with pure enzyme and a water-soluble phosphatidate substrate is applicability to a 96-well format, which may facilitate a large-scale screen of PAP1 inhibitors (or activators) Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.4
-
Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.4 DMSO addition to the reaction mixture results in a dose-dependent inhibition of PAP1 activity, 25% loss of PAP1 activity at a 1% concentration Saccharomyces cerevisiae
3.1.3.4 Phenylglyoxal is an arginine reactive compound, inhibits PAP1 activity in dose-dependent manner Saccharomyces cerevisiae
3.1.3.4 propranolol interacts with the Mg2+-binding site of the enzyme, inhibits PAP1 activity in dose-dependent manner Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.4 Mg2+ colorimetric assay Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.4 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.4 to homogeneity Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.4 5.2
-
pure enzyme, radioactive assay Saccharomyces cerevisiae
3.1.3.4 5.6
-
pure enzyme, colorimetric assay Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.4 dioctanoyl phosphatidic acid + H2O PAP1 activity is linear with respect to the substrate at concentrations between 0.05-0.8 mM Saccharomyces cerevisiae 1,2-dioctanoyl-sn-glycerol + phosphate
-
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Synonyms

EC Number Synonyms Comment Organism
3.1.3.4 3-sn-phosphatidate phosphohydrolase
-
Saccharomyces cerevisiae
3.1.3.4 Mg2+-dependent PA1 phosphatase
-
Saccharomyces cerevisiae
3.1.3.4 Mg2+-dependent phosphatidate phosphatase
-
Saccharomyces cerevisiae
3.1.3.4 pah1
-
Saccharomyces cerevisiae
3.1.3.4 PAP1
-
Saccharomyces cerevisiae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.4 0.2
-
at 30°C, in 50 mM Tris-HCl buffer, pH 7.5, 1 mM MgCl2, and 0.2 mM substrate Saccharomyces cerevisiae propranolol
3.1.3.4 1.3
-
at 30°C, in 50 mM Tris-HCl buffer, pH 7.5, 1 mM MgCl2, and 0.2 mM substrate Saccharomyces cerevisiae Phenylglyoxal