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Literature summary extracted from

  • Chen, R.; Pawlicki, M.; Hamilton, B.; Tolbert, T.
    Enzyme-catalyzed synthesis of a hybrid N-linked oligosaccharide using N-acetylglucosaminyltransferase I (2008), Adv. Synth. Catal., 350, 1689-1695.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.4.1.101 molecular biology production of rare hybrid oligosaccharides for biochemical and structural studies, 100% conversion of oligosaccharide substrate at room temperature, yield of 42% after purification from reaction mixture Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.101 expression of the histidine-tagged catalytic domain in Escherichia coli which is supplemented with mammalian tRNA codons and with mutations to improve intracellular disulfide bond formation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.101 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.101 one Ni2+-NTA chromatography step is sufficient for oligosaccharide synthesis Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.101 0.48
-
 room temperature, 2.7fold excess of UDP-N-acetylglucosamine over the oligosaccharide substrate, 20 mM HEPES, pH 7.5, 150 mM NaCl, 20 mM MnCl2 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.101 UDP-N-acetylglucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide regioselective and stereoselective addition of beta-1,2-N-acetylglucosamine to a high mannose oligosaccharide from yeast, room temperature, 2.7fold excess of UDP-N-acetylglucosamine over the oligosaccharide substrate, 20 mM HEPES, pH 7.5, 150 mM NaCl, 20 mM MnCl2 Homo sapiens UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.101 GnT-I
-
 Homo sapiens
2.4.1.101 N-acetylglucosaminyltransferase I
-
 Homo sapiens