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Literature summary extracted from

  • Barata, L.; Sousa Silva, M.; Schuldt, L.; da Costa, G.; Tomas, A.M.; Ferreira, A.E.; Weiss, M.S.; Ponces Freire, A.; Cordeiro, C.
    Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of glyoxalase I from Leishmania infantum (2010), Acta Crystallogr. Sect. F, 66, 571-574.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.5 expressed in Escherichia coli BL21-Codon-Plus cells Leishmania infantum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.4.1.5 sitting drop vapor diffusion method, using 62% (v/v) 2-methyl-2,4-pentanediol and 100 mM Tris-HCl pH 8.5 Leishmania infantum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 18200
-
 2 * 18200, calculated from amino acid sequence Leishmania infantum

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Leishmania infantum Q2PYM9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.5 Co2+ affinity column chromatography and Superdex S75 gel filtration Leishmania infantum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 methylglyoxal + reduced trypanothione
-
 Leishmania infantum S-D-lactoyltrypanothione
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.5 homodimer 2 * 18200, calculated from amino acid sequence Leishmania infantum

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 Glo1
-
 Leishmania infantum
4.4.1.5 glyoxalase I
-
 Leishmania infantum