Literature summary extracted from

Matsuda, S.; Yokochi, N.; Yoshikane, Y.; Kobayashi, J.; Huy, C.N.; Baba, S.; Kuramitsu, S.; Mikami, B.; Yagi, T.
Crystallization and preliminary X-ray analysis of 4-pyridoxolactonase from Mesorhizobium loti (2009), Acta Crystallogr. Sect. F, 65, 886-889.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.1.27	overexpressed in Escherichia coli BL21 (DE3) cells harbouring plasmid pET21a6805	Mesorhizobium loti

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.1.1.27	by the sitting-drop vapour-diffusion method, at 4°C. Free enzyme to 2.0 A resolution, belongs to the monoclinic space group C2, with unit-cell parameters a = 77.93, b = 38.88, c = 81.60 A, beta = 117.33. The 5-pyridoxolactone-bound enzyme to 1.9 A resolution, belongs to the monoclinic space group C2, with unit-cell parameters a = 86.24, b = 39.35, c = 82.68 A, beta = 118.02	Mesorhizobium loti

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.1.1.27	31000	-	x * 31000, SDS-PAGE	Mesorhizobium loti

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.1.27	Mesorhizobium loti	-	-	-
3.1.1.27	Mesorhizobium loti MAFF303099	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.1.27	recombinant enzyme purified sequentially by gel filtration and ammonium sulfate precipitation	Mesorhizobium loti

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.1.27	4-pyridoxolactone + H2O	-	Mesorhizobium loti	4-pyridoxate	-	?
3.1.1.27	4-pyridoxolactone + H2O	-	Mesorhizobium loti MAFF303099	4-pyridoxate	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.1.1.27	?	x * 31000, SDS-PAGE	Mesorhizobium loti

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.1.27	4-pyridoxolactonase	-	Mesorhizobium loti

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Release 2023.1 (January 2023)