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Literature summary extracted from

  • Kozlov, G.; Nguyen, L.; Pearsall, J.; Gehring, K.
    The structure of phosphate-bound Escherichia coli adenylosuccinate lyase identifies His171 as a catalytic acid (2009), Acta Crystallogr. Sect. F, 65, 857-861.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.2 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.2.2 hanging drop vapor diffusion method, using 1.4 M sodium/potassium phosphate pH 7.5 and 4% (v/v) glycerol Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.2 200000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.2 5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide Escherichia coli
-
 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
4.3.2.2 succinyladenosine monophosphate Escherichia coli
-
 AMP + fumarate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.2 Escherichia coli Q8X737
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.2 Q-Sepharose column chromatography and gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.2 5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide
-
 Escherichia coli 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
4.3.2.2 succinyladenosine monophosphate
-
 Escherichia coli AMP + fumarate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.2.2 homotetramer x-ray crystallography Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.3.2.2 ASL
-
 Escherichia coli