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Literature summary extracted from

  • Bezsudnova, E.Y.; Kovalchuk, M.V.; Mardanov, A.V.; Poliakov, K.M.; Popov, V.O.; Ravin, N.V.; Skryabin, K.G.; Smagin, V.A.; Stekhanova, T.N.; Tikhonova, T.V.
    Overexpression, purification and crystallization of a thermostable DNA ligase from the archaeon Thermococcus sp. 1519 (2009), Acta Crystallogr. Sect. F, 65, 368-371.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expressed in Escherichia coli DLT1270/pRARE-2 cells Thermococcus sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.5.1.1 hanging drop vapor diffusion method, using 35% (v/v) tacsimate pH 7.0 as a precipitant Thermococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Thermococcus sp. C0LJI8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1 Ni-Sepharose column chromatography, Mono beads Q column chromatography, and Superdex 200 gel filtration Thermococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Thermococcus sp. AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 ATP-dependent DNA ligase
-
 Thermococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.1 ATP
-
 Thermococcus sp.