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Literature summary extracted from

  • Zhang, G.; Xu, X.; Li, C.; Ma, B.
    Cloning, expression and reactivating characterization of glycerol dehydratase reactivation factor from Klebsiella pneumoniae XJPD-Li (2009), World J. Microbiol. Biotechnol., 25, 1947-1953.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.30 ATP
-
Klebsiella pneumoniae
4.2.1.30 coenzyme B12
-
Klebsiella pneumoniae
4.2.1.30 DhaF glycerol dehydratase reactivation factor Klebsiella pneumoniae
4.2.1.30 DhaG glycerol dehydratase reactivation factor Klebsiella pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.30 pET-28a(+)-dhaBCEE expressed in Escherichia coli BL21 (DE3) Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.30 glycerol GDHt can be quickly reactivated by glycerol dehydratase reactivation factor to 98% in the presence of ATP, Mg2+ and coenzyme B12. In the first 10 min of the reactivation reaction, the average reactivation rate is 0.18 micromol/min for glycerol-inactivated GDHt Klebsiella pneumoniae
4.2.1.30 O2 GDHt can be quickly reactivated by glycerol dehydratase reactivation factor in the presence of ATP, Mg2+ and coenzyme B12. In the first 10 min of the reactivation reaction, the average reactivation rate is 0.12 micromol/min for O2-inactivated GDHt Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.30 Mg2+
-
Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.30 Klebsiella pneumoniae
-
-
-
4.2.1.30 Klebsiella pneumoniae XJPD-Li
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.30 1,2-propanediol
-
Klebsiella pneumoniae propionaldehyde + H2O
-
?
4.2.1.30 1,2-propanediol
-
Klebsiella pneumoniae XJPD-Li propionaldehyde + H2O
-
?
4.2.1.30 glycerol
-
Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
?
4.2.1.30 glycerol
-
Klebsiella pneumoniae XJPD-Li 3-hydroxypropanal + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.30 GDHt
-
Klebsiella pneumoniae
4.2.1.30 glycerol dehydratase
-
Klebsiella pneumoniae