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Literature summary extracted from

  • Payasi, A.; Sanwal, R.; Sanwal, G.
    Microbial pectate lyases: Characterization and enzymological properties (2009), World J. Microbiol. Biotechnol., 25, 1-14.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.2
-
 Dickeya chrysanthemi
4.2.2.2 expressed in Escherichia coli Bacillus licheniformis
4.2.2.2 expressed in Escherichia coli Niveispirillum irakense

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 Ba2+ strong inhibition Alkalihalobacillus alcalophilus
4.2.2.2 Ba2+ strong inhibition Niveispirillum irakense
4.2.2.2 Co2+ strong inhibition Bacillus pumilus
4.2.2.2 EDTA strong inhibition Bacillus pumilus
4.2.2.2 epicatechin
-
 Dickeya chrysanthemi
4.2.2.2 Mn2+ strong inhibition Alkalihalobacillus alcalophilus
4.2.2.2 Mn2+ weakly inhibits PelA activity at 1 mM Dickeya chrysanthemi
4.2.2.2 additional information Mg2+ and Zn2+ have no effect on Pel-15 activity Bacillus sp. (in: Bacteria)
4.2.2.2 salicylic acid
-
 Dickeya chrysanthemi
4.2.2.2 Sr2+ weakly inhibits PelA activity at 1 mM Dickeya chrysanthemi
4.2.2.2 Zn2+ strong inhibition Niveispirillum irakense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.2 0.05
-
 galacturonan
-
 Dickeya chrysanthemi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ optimum Ca2+ concentration at 0.2 mM Pseudonocardia sp.
4.2.2.2 Ca2+ optimum Ca2+ concentration at 0.2 mM Niveispirillum irakense
4.2.2.2 Ca2+ optimum Ca2+ concentration at 0.5 mM Pseudomonas fluorescens
4.2.2.2 Ca2+ optimum Ca2+ concentration at 0.5 mM Dickeya chrysanthemi
4.2.2.2 Ca2+ optimum Ca2+ concentration at 0.6 mM Bacillus licheniformis
4.2.2.2 Ca2+ optimum Ca2+ concentration at 0.6 mM Fusarium verticillioides
4.2.2.2 Ca2+ optimum Ca2+ concentration at 2.0 mM Bacillus subtilis
4.2.2.2 Ca2+ optimum Ca2+ concentration at 2.0-4.0 mM Cellvibrio japonicus
4.2.2.2 Ca2+ optimum concentration at 0.5 mM Pseudomonas viridiflava
4.2.2.2 Ca2+ optimum concentration at 0.6 mM Bacillus sp. (in: Bacteria)
4.2.2.2 Ca2+ optimum concentration at 0.6 mM Dickeya chrysanthemi
4.2.2.2 Ca2+ optimum concentration at 1.0 mM Pseudomonas marginalis
4.2.2.2 Ca2+ optimum concentration at 1.0 mM, Ca2+ cannot be replaced by Ba2+, Be2+, Sr2+, Mg2+, and monovalent cations for Pel activity Fusarium solani
4.2.2.2 additional information no requirement of Ca2+ Bacillus pumilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 10600
-
 ultracentrifugation Fusarium verticillioides
4.2.2.2 11000
-
 gel filtration Fusarium verticillioides
4.2.2.2 12100
-
 SDS-PAGE Fusarium verticillioides
4.2.2.2 23000
-
 SDS-PAGE Bacillus subtilis
4.2.2.2 31000
-
 SDS-PAGE Pseudonocardia sp.
4.2.2.2 33400
-
 SDS-PAGE Bacillus licheniformis
4.2.2.2 35000
-
 SDS-PAGE Bacillus sp. (in: Bacteria)
4.2.2.2 35000
-
 SDS-PAGE Alkalihalobacillus alcalophilus
4.2.2.2 37300
-
 SDS-PAGE Bacillus pumilus
4.2.2.2 42000
-
 SDS-PAGE Bacillus subtilis
4.2.2.2 42000
-
 SDS-PAGE Clostridium cellulovorans
4.2.2.2 42500
-
 isoform PelA, SDS-PAGE Dickeya chrysanthemi
4.2.2.2 44000
-
 SDS-PAGE Dickeya chrysanthemi
4.2.2.2 44400
-
 SDS-PAGE Niveispirillum irakense
4.2.2.2 50000
-
 SDS-PAGE Bacillus sp. (in: Bacteria)
4.2.2.2 56000
-
 SDS-PAGE Thermobifida fusca
4.2.2.2 68500
-
 SDS-PAGE Cellvibrio japonicus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Alkalihalobacillus alcalophilus
-
-
-
4.2.2.2 Bacillus licheniformis
-
-
-
4.2.2.2 Bacillus pumilus
-
-
-
4.2.2.2 Bacillus pumilus BK2
-
-
-
4.2.2.2 Bacillus sp. (in: Bacteria)
-
-
-
4.2.2.2 Bacillus sp. (in: Bacteria) KSM-P15
-
-
-
4.2.2.2 Bacillus sp. (in: Bacteria) P4-N
-
-
-
4.2.2.2 Bacillus sp. (in: Bacteria) TS 47
-
-
-
4.2.2.2 Bacillus subtilis
-
-
-
4.2.2.2 Bacillus subtilis 168
-
-
-
4.2.2.2 Bacillus subtilis SO113
-
-
-
4.2.2.2 Cellvibrio japonicus
-
-
-
4.2.2.2 Clostridium cellulovorans
-
-
-
4.2.2.2 Dickeya chrysanthemi
-
-
-
4.2.2.2 Dickeya chrysanthemi P04959 isoform PelB
-
4.2.2.2 Dickeya chrysanthemi P0C1A2
-
-
4.2.2.2 Dickeya chrysanthemi P11073 isoform PelC
-
4.2.2.2 Dickeya chrysanthemi P18209 isoform PelD
-
4.2.2.2 Fusarium solani
-
-
-
4.2.2.2 Fusarium verticillioides
-
-
-
4.2.2.2 Niveispirillum irakense
-
-
-
4.2.2.2 Pseudomonas fluorescens
-
-
-
4.2.2.2 Pseudomonas marginalis
-
-
-
4.2.2.2 Pseudomonas viridiflava
-
-
-
4.2.2.2 Pseudonocardia sp.
-
-
-
4.2.2.2 Thermobifida fusca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2
-
 Niveispirillum irakense
4.2.2.2 affinity column chromatography and gel filtration Fusarium verticillioides
4.2.2.2 Ni-NTA column chromatography Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 citrus pectin
-
 Bacillus pumilus unsaturated tetragalacturonate
-
 ?
4.2.2.2 citrus pectin
-
 Bacillus pumilus BK2 unsaturated tetragalacturonate
-
 ?
4.2.2.2 galacturonan
-
 Dickeya chrysanthemi ?
-
 ?
4.2.2.2 pectin
-
 Bacillus subtilis ?
-
 ?
4.2.2.2 pectin
-
 Pseudomonas fluorescens ?
-
 ?
4.2.2.2 pectin
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
4.2.2.2 pectin
-
 Bacillus licheniformis ?
-
 ?
4.2.2.2 pectin
-
 Dickeya chrysanthemi ?
-
 ?
4.2.2.2 pectin
-
 Pseudonocardia sp. ?
-
 ?
4.2.2.2 pectin
-
 Pseudomonas viridiflava ?
-
 ?
4.2.2.2 pectin
-
 Pseudomonas marginalis ?
-
 ?
4.2.2.2 pectin
-
 Fusarium solani ?
-
 ?
4.2.2.2 pectin
-
 Thermobifida fusca ?
-
 ?
4.2.2.2 pectin
-
 Fusarium verticillioides ?
-
 ?
4.2.2.2 pectin
-
 Alkalihalobacillus alcalophilus ?
-
 ?
4.2.2.2 pectin
-
 Cellvibrio japonicus ?
-
 ?
4.2.2.2 pectin
-
 Niveispirillum irakense ?
-
 ?
4.2.2.2 pectin
-
 Clostridium cellulovorans ?
-
 ?
4.2.2.2 pectin
-
 Bacillus subtilis 168 ?
-
 ?
4.2.2.2 pectin
-
 Bacillus subtilis SO113 ?
-
 ?
4.2.2.2 pectin
-
 Bacillus sp. (in: Bacteria) TS 47 ?
-
 ?
4.2.2.2 pectin
-
 Bacillus sp. (in: Bacteria) KSM-P15 ?
-
 ?
4.2.2.2 pectin
-
 Bacillus sp. (in: Bacteria) P4-N ?
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Bacillus subtilis unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Pseudomonas fluorescens unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Bacillus sp. (in: Bacteria) unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Dickeya chrysanthemi unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Pseudonocardia sp. unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Pseudomonas viridiflava unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Pseudomonas marginalis unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Fusarium solani unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Thermobifida fusca unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Fusarium verticillioides unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Alkalihalobacillus alcalophilus unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Cellvibrio japonicus unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Niveispirillum irakense unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Clostridium cellulovorans unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Bacillus subtilis 168 unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Bacillus subtilis SO113 unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Bacillus sp. (in: Bacteria) TS 47 unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Bacillus sp. (in: Bacteria) KSM-P15 unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Bacillus sp. (in: Bacteria) P4-N unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Bacillus pumilus unsaturated tetragalacturonate
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Bacillus pumilus BK2 unsaturated tetragalacturonate
-
 ?
4.2.2.2 polygalacturonate pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction Bacillus licheniformis unsaturated trigalacturonate + unsaturated oligogalacturonates major product ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.2 Pel
-
 Bacillus subtilis
4.2.2.2 Pel
-
 Bacillus sp. (in: Bacteria)
4.2.2.2 Pel
-
 Pseudomonas viridiflava
4.2.2.2 Pel
-
 Pseudomonas marginalis
4.2.2.2 Pel
-
 Fusarium solani
4.2.2.2 Pel
-
 Thermobifida fusca
4.2.2.2 Pel-15
-
 Bacillus sp. (in: Bacteria)
4.2.2.2 PelA
-
 Bacillus subtilis
4.2.2.2 PelA
-
 Pseudomonas fluorescens
4.2.2.2 PelA
-
 Bacillus sp. (in: Bacteria)
4.2.2.2 PelA
-
 Bacillus licheniformis
4.2.2.2 PelA
-
 Bacillus pumilus
4.2.2.2 PelA
-
 Pseudonocardia sp.
4.2.2.2 PelA
-
 Fusarium verticillioides
4.2.2.2 PelA
-
 Alkalihalobacillus alcalophilus
4.2.2.2 PelA
-
 Cellvibrio japonicus
4.2.2.2 PelA
-
 Niveispirillum irakense
4.2.2.2 PelA
-
 Clostridium cellulovorans
4.2.2.2 PelA
-
 Dickeya chrysanthemi
4.2.2.2 PelA isoform Dickeya chrysanthemi
4.2.2.2 PelB isoform Dickeya chrysanthemi
4.2.2.2 pelC isoform Dickeya chrysanthemi
4.2.2.2 PelD isoform Dickeya chrysanthemi
4.2.2.2 PelE isoform Dickeya chrysanthemi
4.2.2.2 PelI
-
 Dickeya chrysanthemi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 40
-
-
 Bacillus subtilis
4.2.2.2 40
-
-
 Niveispirillum irakense
4.2.2.2 45
-
-
 Alkalihalobacillus alcalophilus
4.2.2.2 50
-
-
 Clostridium cellulovorans
4.2.2.2 50
-
-
 Dickeya chrysanthemi
4.2.2.2 50
-
 isoform PelD Dickeya chrysanthemi
4.2.2.2 50
-
 isoform PelE Dickeya chrysanthemi
4.2.2.2 55
-
 isoform PelA Dickeya chrysanthemi
4.2.2.2 60
-
-
 Thermobifida fusca
4.2.2.2 60
-
 isoform PelB Dickeya chrysanthemi
4.2.2.2 60
-
 isoform PelC Dickeya chrysanthemi
4.2.2.2 62
-
-
 Cellvibrio japonicus
4.2.2.2 65
-
-
 Bacillus subtilis
4.2.2.2 65
-
-
 Bacillus licheniformis
4.2.2.2 70
-
-
 Bacillus sp. (in: Bacteria)
4.2.2.2 70
-
-
 Bacillus pumilus
4.2.2.2 70
-
-
 Pseudonocardia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 8
-
-
 Bacillus sp. (in: Bacteria)
4.2.2.2 8
-
-
 Clostridium cellulovorans
4.2.2.2 8
-
 isoform PelE Dickeya chrysanthemi
4.2.2.2 8.4
-
-
 Bacillus subtilis
4.2.2.2 8.5
-
-
 Bacillus licheniformis
4.2.2.2 8.5
-
-
 Bacillus pumilus
4.2.2.2 8.5
-
-
 Fusarium verticillioides
4.2.2.2 8.5
-
 isoform PelA Dickeya chrysanthemi
4.2.2.2 8.6
-
-
 Dickeya chrysanthemi
4.2.2.2 8.8
-
 isoform PelD Dickeya chrysanthemi
4.2.2.2 9
-
-
 Niveispirillum irakense
4.2.2.2 9 10
-
 Alkalihalobacillus alcalophilus
4.2.2.2 9.2
-
 isoform PelC Dickeya chrysanthemi
4.2.2.2 10
-
-
 Bacillus subtilis
4.2.2.2 10
-
-
 Cellvibrio japonicus
4.2.2.2 10.3
-
-
 Pseudonocardia sp.
4.2.2.2 10.5
-
-
 Thermobifida fusca
4.2.2.2 11.5
-
-
 Bacillus sp. (in: Bacteria)

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.2 Thermobifida fusca isoelectric focusing
-
 4.2
4.2.2.2 Dickeya chrysanthemi isoelectric focusing
-
 4.6
4.2.2.2 Dickeya chrysanthemi isoform PelA, isoelectric focusing
-
 4.6
4.2.2.2 Bacillus sp. (in: Bacteria) isoelectric focusing
-
 5.3
4.2.2.2 Niveispirillum irakense isoelectric focusing
-
 6.2
4.2.2.2 Pseudomonas fluorescens minor pI, isoelectric focusing
-
 6.7
4.2.2.2 Dickeya chrysanthemi isoform PelB, isoelectric focusing
-
 7.9
4.2.2.2 Dickeya chrysanthemi isoform PelC, isoelectric focusing
-
 8.2
4.2.2.2 Bacillus pumilus isoelectric focusing
-
 8.5
4.2.2.2 Bacillus subtilis isoelectric focusing
-
 8.6
4.2.2.2 Fusarium verticillioides isoelectric focusing
-
 9.1
4.2.2.2 Bacillus subtilis isoelectric focusing
-
 9.6
4.2.2.2 Bacillus sp. (in: Bacteria) isoelectric focusing
-
 9.6
4.2.2.2 Pseudomonas viridiflava isoelectric focusing
-
 9.7
4.2.2.2 Dickeya chrysanthemi isoform PelD, isoelectric focusing
-
 9.8
4.2.2.2 Pseudonocardia sp. isoelectric focusing
-
 10
4.2.2.2 Pseudomonas fluorescens major pI, isoelectric focusing
-
 10
4.2.2.2 Dickeya chrysanthemi isoform PelE, isoelectric focusing
-
 10.3