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Literature summary extracted from

  • Yamamoto, F.; Yamamoto, M.; Blancher, A.
    Generation of histo-blood group B transferase by replacing the N-acetyl-D-galactosamine recognition domain of human A transferase with the galactose-recognition domain of evolutionarily related murine alpha1,3-galactosyltransferase (2009), Transfusion, 50, 622-630.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.37 expression of wild-type and chimeric mutant enzyme in HeLa cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.37 additional information generation of a structural chemira of histo-blood group B transferase, a B type transferase, by replacing the N-acetyl-D-galactosamine recognition domain of human type A transferase with the galactose-recognition domain of evolutionarily related murine alpha1,3-galactosyltransferase, leading to functional conversion from human A to B transferase activity, overview. When the glycine 268 of the A transferase is substituted by the alanine of the B transferase, the construct expresses an enzyme with weak B transferase activity, but when the alanine 268 of B transferase is substituted by the glycine of A transferase, the construct expresses an enzyme with strong A and B transferase activity Homo sapiens
2.4.1.40 additional information generation of a chimeric histo-blood group B transferase by replacing the N-acetyl-D-galactosamine recognition domain of human A transferase with the galactose-recognition domain of evolutionarily related murine alpha1,3-galactosyltransferase, the chimeric mutant shows a functional conversion from A to B transferase activity, overview. The AT-MluI-GT construct, which expresses the chimera between the long N-terminal sequence up to the MluI site of human A transferase, and the short C-terminal sequence up to the MluI site of murine a1-3Gal transferase, produces the antigens that react with both the anti-A and the Griffonia simplicifolia Lectin I, GSL-IA4, lectin Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.37 additional information Homo sapiens histo-blood group B transferase transfers alpha-D-galatose to the antigen,H, while the B type enzyme transfers N-acetylgalactosamine, overview ?
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 ?
2.4.1.37 UDP-galactose + blood group antigen H Homo sapiens the alpha1,3-galactosyl epitope is a major xenotransplant antigen UDP + alpha-D-galactosyl-blood group antigen H
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 ?
2.4.1.40 UDP-N-acetyl-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose Homo sapiens substrate is the histo-blood H group antigen UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactose product is the histo-blood A group antigen ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.37 Homo sapiens
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-
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2.4.1.40 Homo sapiens
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 A transferase
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.37 additional information histo-blood group B transferase transfers alpha-D-galatose to the antigen,H, while the B type enzyme transfers N-acetylgalactosamine, overview Homo sapiens ?
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 ?
2.4.1.37 UDP-galactose + blood group antigen H the alpha1,3-galactosyl epitope is a major xenotransplant antigen Homo sapiens UDP + alpha-D-galactosyl-blood group antigen H
-
 ?
2.4.1.37 UDP-galactose + blood group antigen H alpha-L-fucosyl-(1,2)-D-galactosyl-antigen H Homo sapiens UDP + alpha-D-galactosyl-blood group antigen H alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactosyl-antigen H ?
2.4.1.40 UDP-N-acetyl-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose substrate is the histo-blood H group antigen Homo sapiens UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactose product is the histo-blood A group antigen ?
2.4.1.40 UDP-N-acetyl-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose substrate is the histo-blood H group antigen. Arg176 of the enzyme is not important in determining the sugar specificity of enzyme, while Gly235 is influential and Leu266 and Gly268 are crucial in the determination of GalNAc/galactose specificity Homo sapiens UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactose product is the histo-blood A group antigen ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.37 B transferase
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 Homo sapiens
2.4.1.37 histo-blood group B transferase
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 Homo sapiens
2.4.1.40 A transferase
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 Homo sapiens
2.4.1.40 ABO
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 Homo sapiens
2.4.1.40 More the A/B transferases, ABO, belong to the GT6 enzyme family Homo sapiens