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Literature summary extracted from

  • Sciandrello, G.; Mauro, M.; Caradonna, F.; Catanzaro, I.; Saverini, M.; Barbata, G.
    Acrylamide catalytically inhibits topoisomerase II in V79 cells (2010), Toxicol. In Vitro, 24, 830-834.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.2 Acrylamide acrylamide is a catalytic inhibitor, no enzyme poison, it antagonizes the effect of etoposide, a topoisomerase II poison, and inhibits the enzyme in vivo in V-79 cells. Acrylamide reduces the level of catalytically active cellular topoisomerase II available for the action of etoposide. Assay in presence of DTT Cricetulus griseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.2.2 nucleus
-
 Cricetulus griseus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.2 Mg2+
-
 Cricetulus griseus

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Cricetulus griseus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.2.2 V-79 cell
-
 Cricetulus griseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.2 additional information the enzyme converts kinetoplast DNA to the decatenated form. Cleavage of pBR322 DNA Cricetulus griseus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.2.2 Topoisomerase II
-
 Cricetulus griseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.2.2 37
-
 assay at Cricetulus griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.2.2 8
-
 assay at Cricetulus griseus

Cofactor

EC Number Cofactor Comment Organism Structure
5.6.2.2 ATP
-
 Cricetulus griseus