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Literature summary extracted from

  • Mittasch, J.; Mikolajewski, S.; Breuer, F.; Strack, D.; Milkowski, C.
    Genomic microstructure and differential expression of the genes encoding UDP-glucose:sinapate glucosyltransferase (UGT84A9) in oilseed rape (Brassica napus) (2010), Theor. Appl. Genet., 120, 1485-1500.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.120 agriculture the genes encoding this enzyme are considered as potential targets for molecular breeding of low sinapine oilseed rape Brassica napus var. napus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.120 heterologous expression in Escherichia coli strain BL21 Brassica napus var. napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.120 cytoplasm
-
 Brassica napus var. napus 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.120 UDP-alpha-D-glucose + sinapate Brassica napus var. napus
-
 UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.120 Brassica napus var. napus C1L318 UGT84A9a, gene locus belongs to sequence type 1, genome harbours 4 distinct gene loci; cv. Express
-
2.4.1.120 Brassica napus var. napus C1L319 UGT84A9b, gene locus belongs to sequence type 1, genome harbours 4 distinct gene loci; cv. Express
-
2.4.1.120 Brassica napus var. napus C1L320 UGT84A9c, gene locus belongs to sequence type 2, genome harbours 4 distinct gene loci; cv. Express
-
2.4.1.120 Brassica napus var. napus C1L321 UGT84A9d, gene locus belongs to sequence type 2, genome harbours 4 distinct gene loci; cv. Express
-
2.4.1.120 Brassica napus var. napus Q9FYU7 UGT84A9-1, sequence type 1; cv. Express
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.120 flower
-
 Brassica napus var. napus
-
2.4.1.120 seed at cotyledon stage and at well-developed mature embryo stage Brassica napus var. napus
-
2.4.1.120 seedling 2-day-old Brassica napus var. napus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.120 UDP-alpha-D-glucose + sinapate
-
 Brassica napus var. napus UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.120 SGT
-
 Brassica napus var. napus
2.4.1.120 UDP-glucose:sinapate glucosyltransferase
-
 Brassica napus var. napus
2.4.1.120 UGT84A9
-
 Brassica napus var. napus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.120 30
-
 assay at Brassica napus var. napus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.120 6
-
 assay at Brassica napus var. napus

Expression

EC Number Organism Comment Expression
2.4.1.120 Brassica napus var. napus the genomic microstructures reveal the presence of several transposon-derived sequence elements at all 4 UGT84A9 loci which underlie increased reorganization, weakly expressed sequence type 2 orthologs UGT84A9c and d in the Brassica genomes might indicate that these gene copies are necessary for genetic robustness additional information
2.4.1.120 Brassica napus var. napus Brassicus napus harbors 2 distinguishable sequence types of the UGT84A9 gene designated as UGT84A9-1 and UGT84A9-2, UGT84A9-1 is the predominantly expressed variant, which is significantly up-regulated during the seed filling phase, when sinapate ester biosynthesis exhibits strongest activity up

General Information

EC Number General Information Comment Organism
2.4.1.120 physiological function key enzyme for sinapate ester biosynthesis, the glucosyltransferase UGT84A9 catalyzes the formation of 1-O-sinapoyl-D-glucose, which feeds as acyl donor into a broad range of accumulating sinapate esters, including the major antinutritive seed component sinapoylcholine (sinapine) Brassica napus var. napus
2.4.1.120 physiological function key enzyme for sinapate ester biosynthesis, the glucosyltransferase UGT84A9 catalyzes the formation of 1-O-sinapoyl-D-glucose, which feeds as acyl donor into a broad range of accumulating sinapate esters, including the major antinutritive seed component sinapoylcholine (sinapine) Brassica napus var. napus