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Literature summary extracted from
- Quinoline 2-oxidoreductase and 2-oxo-1,2-dihydroquinoline 5,6-dioxygenase from Comamonas testosteroni 63. The first two enzymes in quinoline and 3-methylquinoline degradation (1995), Eur. J. Biochem., 232, 536-544.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.12.16 | dithioerythritol, dithiothreitol and 2-oxo-1,2-dihydroquinoline stabilize | Comamonas testosteroni |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.16 | 1,10-phenanthroline | 0.5 mM, 82% loss of activity | Comamonas testosteroni |  |
| 1.14.12.16 | 4-hydroxymercuribenzoate | 0.1 mM, complete loss of activity | Comamonas testosteroni | |
| 1.14.12.16 | Acriflavin | 0.5 mM, 69% loss of activity | Comamonas testosteroni | |
| 1.14.12.16 | Cu2+ | - |
Comamonas testosteroni | |
| 1.14.12.16 | diethyldithiocarbaminate | inhibits after a prolonged incubation time | Comamonas testosteroni | |
| 1.14.12.16 | EDTA | inhibits after a prolonged incubation time | Comamonas testosteroni | |
| 1.14.12.16 | iodoacetate | 2 mM, complete loss of activity | Comamonas testosteroni | |
| 1.14.12.16 | NaCl | 0.2 M, 50% loss of activity | Comamonas testosteroni | |
| 1.14.12.16 | Quinacrine | 0.5 mM, 15% loss of activity | Comamonas testosteroni | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.99.17 | Fe | - |
Pseudomonas putida | |
| 1.3.99.17 | Fe | - |
Rhodococcus sp. | |
| 1.3.99.17 | Fe | enzyme contains 7.7 mol iron and 7.5 mol acid labile sulfur per mol of enzyme | Comamonas testosteroni | |
| 1.3.99.17 | Mo | contains 1.6 mol molybdenum per mol of enzyme | Comamonas testosteroni | |
| 1.3.99.17 | Mo | molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor | Pseudomonas putida | |
| 1.3.99.17 | Mo | molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor | Comamonas testosteroni | |
| 1.3.99.17 | Mo | molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor | Rhodococcus sp. | |
| 1.14.12.16 | Fe2+ | enhances activity 1.5fold | Comamonas testosteroni | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.99.17 | 22000 | - |
x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE | Comamonas testosteroni |
| 1.3.99.17 | 32000 | - |
x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE | Comamonas testosteroni |
| 1.3.99.17 | 87000 | - |
x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE | Comamonas testosteroni |
| 1.3.99.17 | 360000 | - |
gel filtration | Comamonas testosteroni |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.99.17 | (3-Methyl)-quinoline + acceptor + H2O | Comamonas testosteroni | first step of 3-methylquinoline degradation | ? | - |
? | |
| 1.3.99.17 | Quinoline + acceptor + H2O | Comamonas testosteroni | first step of quinoline degradation | ? | - |
? | |
| 1.14.12.16 | additional information | Comamonas testosteroni | second enzyme in the pathway of quinoline and 3-methylquinoline degradation | ? | - |
? | |
| 1.14.12.16 | additional information | Comamonas testosteroni 63 | second enzyme in the pathway of quinoline and 3-methylquinoline degradation | ? | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.14.12.16 | Ethanol | 5%, decreases enzyme activity to 44% | Comamonas testosteroni |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.99.17 | Comamonas testosteroni | - |
- |
- |
| 1.3.99.17 | Comamonas testosteroni 63 | - |
- |
- |
| 1.3.99.17 | Pseudomonas putida | - |
- |
- |
| 1.3.99.17 | Pseudomonas putida 86 | - |
- |
- |
| 1.3.99.17 | Rhodococcus sp. | - |
- |
- |
| 1.3.99.17 | Rhodococcus sp. B1 | - |
- |
- |
| 1.14.12.16 | Comamonas testosteroni | - |
- |
- |
| 1.14.12.16 | Comamonas testosteroni 63 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.99.17 | - |
Comamonas testosteroni |
| 1.14.12.16 | partial | Comamonas testosteroni |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.99.17 | 4.8 | - |
- |
Comamonas testosteroni |
| 1.14.12.16 | 0.125 | - |
- |
Comamonas testosteroni |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.99.17 | (3-methyl)-quinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida | (3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet | - |
? | |
| 1.3.99.17 | (3-methyl)-quinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Comamonas testosteroni | (3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet | - |
? | |
| 1.3.99.17 | (3-methyl)-quinoline + acceptor | 61% of the activity with quinoline | Comamonas testosteroni | (3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet | - |
? | |
| 1.3.99.17 | (3-methyl)-quinoline + acceptor | 22% of the activity with quinoline | Pseudomonas putida | (3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet | - |
? | |
| 1.3.99.17 | (3-Methyl)-quinoline + acceptor + H2O | first step of 3-methylquinoline degradation | Comamonas testosteroni | ? | - |
? | |
| 1.3.99.17 | 4-chloroquinoline + p-iodonitrotetrazolium violet | 5% of the activity with quinoline | Comamonas testosteroni | ? | - |
? | |
| 1.3.99.17 | 4-chloroquinoline + p-iodonitrotetrazolium violet | 8% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
| 1.3.99.17 | 4-methylquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida | ? | - |
? | |
| 1.3.99.17 | 4-methylquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Comamonas testosteroni | ? | - |
? | |
| 1.3.99.17 | 4-methylquinoline + acceptor | 21% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
| 1.3.99.17 | 4-methylquinoline + acceptor | 12% of the activity with quinoline | Comamonas testosteroni | ? | - |
? | |
| 1.3.99.17 | 6-hydroxyquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida | ? | - |
? | |
| 1.3.99.17 | 6-hydroxyquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Comamonas testosteroni | ? | - |
? | |
| 1.3.99.17 | 6-hydroxyquinoline + acceptor | 58% of the activity with quinoline | Comamonas testosteroni | ? | - |
? | |
| 1.3.99.17 | 6-hydroxyquinoline + acceptor | 25% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
| 1.3.99.17 | 6-hydroxyquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida 86 | ? | - |
? | |
| 1.3.99.17 | 6-hydroxyquinoline + acceptor | 25% of the activity with quinoline | Pseudomonas putida 86 | ? | - |
? | |
| 1.3.99.17 | 8-chloroquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida | ? | - |
? | |
| 1.3.99.17 | 8-chloroquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Comamonas testosteroni | ? | - |
? | |
| 1.3.99.17 | 8-chloroquinoline + acceptor | 55% of the activity with quinoline | Comamonas testosteroni | ? | - |
? | |
| 1.3.99.17 | 8-chloroquinoline + acceptor | 29% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
| 1.3.99.17 | 8-chloroquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida 86 | ? | - |
? | |
| 1.3.99.17 | 8-chloroquinoline + acceptor | 29% of the activity with quinoline | Pseudomonas putida 86 | ? | - |
? | |
| 1.3.99.17 | 8-methylquinoline + acceptor | 32% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
| 1.3.99.17 | 8-methylquinoline + acceptor | 54% of the activity with quinoline | Comamonas testosteroni | ? | - |
? | |
| 1.3.99.17 | isoquinoline + p-iodonitrotetrazolium violet | 75% of the activity with quinoline | Comamonas testosteroni | ? | - |
? | |
| 1.3.99.17 | quinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
| 1.3.99.17 | quinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Comamonas testosteroni | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
| 1.3.99.17 | quinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida 86 | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
| 1.3.99.17 | Quinoline + acceptor + H2O | first step of quinoline degradation | Comamonas testosteroni | ? | - |
? | |
| 1.14.12.16 | 2-Oxo-1,2-dihydroquinoline + NADH + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| 1.14.12.16 | 2-Oxo-1,2-dihydroquinoline + NADH + O2 | - |
Comamonas testosteroni 63 | ? | - |
? | |
| 1.14.12.16 | 3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2 | NADH cannot be replaced by NADPH | Comamonas testosteroni | 5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+ | - |
? | |
| 1.14.12.16 | 3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2 | NADH cannot be replaced by NADPH | Comamonas testosteroni 63 | 5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+ | - |
? | |
| 1.14.12.16 | 6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| 1.14.12.16 | 6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2 | - |
Comamonas testosteroni 63 | ? | - |
? | |
| 1.14.12.16 | 8-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| 1.14.12.16 | 8-Hydroxyquinoline + NADH + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| 1.14.12.16 | 8-Hydroxyquinoline + NADH + O2 | - |
Comamonas testosteroni 63 | ? | - |
? | |
| 1.14.12.16 | additional information | second enzyme in the pathway of quinoline and 3-methylquinoline degradation | Comamonas testosteroni | ? | - |
? | |
| 1.14.12.16 | additional information | second enzyme in the pathway of quinoline and 3-methylquinoline degradation | Comamonas testosteroni 63 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.3.99.17 | ? | - |
Pseudomonas putida |
| 1.3.99.17 | ? | - |
Rhodococcus sp. |
| 1.3.99.17 | ? | x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE | Comamonas testosteroni |
| 1.3.99.17 | More | the N-termini of the beta-subunits show striking similarities | Pseudomonas putida |
| 1.3.99.17 | More | the N-termini of the beta-subunits show striking similarities | Comamonas testosteroni |
| 1.3.99.17 | More | the N-termini of the beta-subunits show striking similarities | Rhodococcus sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.16 | 30 | - |
5 min, 30% loss of activity | Comamonas testosteroni |
| 1.14.12.16 | 65 | - |
5 min, complete loss of activity | Comamonas testosteroni |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.16 | 7.3 | - |
- |
Comamonas testosteroni |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.99.17 | FAD | - |
Pseudomonas putida | |
| 1.3.99.17 | FAD | enzyme contains 1.8 mol FAD per mol of enzyme | Comamonas testosteroni | |
| 1.3.99.17 | FAD | enzyme contains 1.8 mol FAD per mol of enzyme | Rhodococcus sp. | |
| 1.3.99.17 | molybdopterin | molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor | Pseudomonas putida | |
| 1.3.99.17 | molybdopterin | molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor | Comamonas testosteroni | |
| 1.3.99.17 | molybdopterin | molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor | Rhodococcus sp. | |
| 1.14.12.16 | NADH | required, cannot be replaced by NADPH | Comamonas testosteroni | |
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