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Literature summary extracted from
- Expression, purification and characterization of a thermostable pectate lyase from Thermotoga maritima (2009), Sheng Wu Gong Cheng Xue Bao, 25, 223-229.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.2.2 | expressed in Escherichia coli JM109 cells | Thermotoga maritima |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.2.2 | 0.11 | - |
Polygalacturonate | - |
Thermotoga maritima |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.2 | Ca2+ | PelC is dependent on Ca2+ for activity and stability | Thermotoga maritima | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 43000 | - |
recombinant enzyme, SDS-PAGE | Thermotoga maritima |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.2 | Thermotoga maritima | - |
- |
- |
| 4.2.2.2 | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.2 | polygalacturonate | - |
Thermotoga maritima | unsaturated oligogalacturonides | - |
? | |
| 4.2.2.2 | polygalacturonate | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | unsaturated oligogalacturonides | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.2.2 | pectate lyase C | - |
Thermotoga maritima |
| 4.2.2.2 | pelC | - |
Thermotoga maritima |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 90 | - |
- |
Thermotoga maritima |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 60 | 95 | the enzyme keeps stable, possesses a high level of activity at 60°C and a half-life of almost 2 h at 95°C | Thermotoga maritima |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 8.5 | - |
- |
Thermotoga maritima |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 8.2 | 9.8 | - |
Thermotoga maritima |
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