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Literature summary extracted from

  • Cubrilo, S.; Babic, F.; Douthwaite, S.; Maravic Vlahovicek, G.
    The aminoglycoside resistance methyltransferase Sgm impedes RsmF methylation at an adjacent rRNA nucleotide in the ribosomal A site (2009), RNA, 15, 1492-1497.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.179 introduction of a recombinant version of the Sgm methyltransferase gene from Micromonospora zionensis into an Escherichia coli strain that has a full complement of housekeeping methyltransferases. Analyses of the 16S rRNA shows that the m5C1407-specific housekeeping methyltransferase RsmF (YebU) is outcompeted by Sgm as the resistance methyltransferase gains access to its own m7G1405 target on the 30S ribosomal subunit. A single amino acid change in Sgm, which lowers the level of conferred resistance, reduces the ability of Sgm to interfere with RsmF methylation on the 30S subunit Micromonospora zionensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.178 additional information introduction of a recombinant version of the Sgm methyltransferase gene from Micromonospora zionensis into an Escherichia coli strain that has a full complement of housekeeping methyltransferases. Analyses of the 16S rRNA shows that the m5C1407-specific housekeeping methyltransferase RsmF (YebU) is outcompeted by Sgm as the resistance methyltransferase gains access to its own m7G1405 target on the 30S ribosomal subunit. A single amino acid change in Sgm, which lowers the level of conferred resistance, reduces the ability of Sgm to interfere with RsmF methylation on the 30S subunit Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.179 S-adenosyl-L-methionine + guanine1405 in 16S rRNA Micromonospora zionensis
-
 S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.178 Escherichia coli P76273
-
-
2.1.1.179 Micromonospora zionensis Q7M0R2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.179 S-adenosyl-L-methionine + guanine1405 in 16S rRNA
-
 Micromonospora zionensis S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.178 RsmF
-
 Escherichia coli
2.1.1.179 Sgm methyltransferase
-
 Micromonospora zionensis