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Literature summary extracted from

  • Araya, M.A.; Tantalean, J.C.; Perez, J.M.; Fuentes, D.E.; Calderon, I.L.; Saavedra, C.P.; Burra, R.; Chasteen, T.G.; Vasquez, C.C.
    Cloning, purification and characterization of Geobacillus stearothermophilus V uroporphyrinogen-III C-methyltransferase: evaluation of its role in resistance to potassium tellurite in Escherichia coli (2009), Res. Microbiol., 160, 125-133.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.107 gene cobA, expression in Escherichia coli strain JM109(DE3), the recombinant enzyme renders the bacteria morre resistant to potassium tellurite and potassium tellurate Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.107 G12A site-directed mutagenesis of cobA abolishes the tellurite resistance of the mesophilic, heterologous host Escherichia coli and the SUMT activity in vitro. Cells overexpressing SUMT G12A show 7fold less tolerance to K2TeO3 as compared to that exhibit by cells expressing the wild-type methyltransferase Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.107 30000
-
 2 * 30000, recombinant enzyme, SDS-PAGE Geobacillus stearothermophilus
2.1.1.107 60000
-
 recombinant enzyme, native PAGE and gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.107 Geobacillus stearothermophilus Q6TA16 gene cobA
-
2.1.1.107 Geobacillus stearothermophilus V Q6TA16 gene cobA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.107 uroporphyrinogen III + S-adenosyl-L-methionine the enzyme binds S-adenosyl-L-methionine and catalyzes the production of III methyl uroporphyrinogen in vitro Geobacillus stearothermophilus precorrin-2 + S-adenosyl-L-homocysteine
-
 ?
2.1.1.107 uroporphyrinogen III + S-adenosyl-L-methionine the enzyme binds S-adenosyl-L-methionine and catalyzes the production of III methyl uroporphyrinogen in vitro Geobacillus stearothermophilus V precorrin-2 + S-adenosyl-L-homocysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.107 homodimer 2 * 30000, recombinant enzyme, SDS-PAGE Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.107 SUMT
-
 Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.107 S-adenosyl-L-methionine
-
 Geobacillus stearothermophilus

General Information

EC Number General Information Comment Organism
2.1.1.107 physiological function the role of SUMT methyltransferase in tellurite(ate) detoxification is not related to tellurium volatilization Geobacillus stearothermophilus