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Literature summary extracted from

  • Herouet-Guicheney, C.; Rouquie, D.; Freyssinet, M.; Currier, T.; Martone, A.; Zhou, J.; Bates, E.E.; Ferullo, J.M.; Hendrickx, K.; Rouan, D.
    Safety evaluation of the double mutant 5-enol pyruvylshikimate-3-phosphate synthase (2mEPSPS) from maize that confers tolerance to glyphosate herbicide in transgenic plants (2009), Regul. Toxicol. Pharmacol., 54, 143-153.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19 expressed in Escherichia coli Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.19 T103I/P107S double mutant (47 kDa) which is widely used by genetic engineering to confer glyphosate tolerant properties, to various crops such as corn, cotton, canola, and soybean is assessed in terms of safety aspects: The expressed protein is innocuous. The double mutant enzyme does not possess any of the properties associated with known toxins or allergens, a rapid degradation in simulated gastric and intestinal fluids, and no adverse effects in mice after intravenousor oral administration is shown Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19 using an anion exchange Q-Sepharose column, ammonium sulfate precipitation using an equilibrated phenyl Sepharose column Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Zea mays phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.19 EPSPS
-
 Zea mays