Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Davlieva, M.; Shamoo, Y.
    Crystal structure of a trimeric archaeal adenylate kinase from the mesophile Methanococcus maripaludis with an unusually broad functional range and thermal stability (2010), Proteins, 78, 357-364.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.3 expressed in Escherichia coli Rosetta cells Methanococcus maripaludis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.3 sitting drop vapor diffusion method, using in 3.4 M ammonium chloride, 0.1 M sodium acetate (pH 4.7), and 3% ethylene glycol (v/v), at 22°C Methanococcus maripaludis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.3 0.00537
-
 ATP at 30°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis
2.7.4.3 0.00959
-
 AMP at 30°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis
2.7.4.3 0.01659
-
 ATP at 40°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis
2.7.4.3 0.0372
-
 AMP at 40°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis
2.7.4.3 0.05547
-
 AMP at 60°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis
2.7.4.3 0.07389
-
 AMP at 50°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis
2.7.4.3 0.09471
-
 ATP at 50°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis
2.7.4.3 0.1349
-
 ATP at 60°C, in 25 mM phosphate buffer (pH 7.2), 5 mM MgCl2 Methanococcus maripaludis

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Methanococcus maripaludis Q6LYG0 strain ATCC 43000D
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.3 HiTrap Q-XL Sepharose column chromatography, Affi-Gel blue resin colum chromatography, and Superdex-200 gel filtration Methanococcus maripaludis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ATP + AMP
-
 Methanococcus maripaludis ADP + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.3 trimer x-ray crystallography Methanococcus maripaludis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.4.3 30 60 excellent activity over a broad temperature range of 30-60°C Methanococcus maripaludis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.3 74
-
 the melting temperature is at 74°C Methanococcus maripaludis