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Literature summary extracted from
- Crystal structure of a putative methylmalonyl-coenzyme a epimerase from Thermoanaerobacter tengcongensis at 2.0 Å resolution (2009), Proteins, 77, 994-999.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.99.1 | recombinant protein is expressed in BL21(DE3) Escherichia coli cells | Caldanaerobacter subterraneus subsp. tengcongensis |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.1.99.1 | at 2.0 A resolutionm, crystals of recombinant enzyme are grown at 20°C using the hanging-drop, vapor-diffusion method, drops consisted of 2 microl of protein solution and 2 microl of mother liquor (0.1 M citric acid, pH 2.6, 1.6 M (NH4)2SO4), crystals are obtained after 7-8 days growth, native and derivative crystals are soaked in 2 M Li2SO4 | Caldanaerobacter subterraneus subsp. tengcongensis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.99.1 | Co2+ | enzyme is metal-dependent enzyme, in the active site, metal binding site binds the coenzyme Co2+ | Caldanaerobacter subterraneus subsp. tengcongensis |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.99.1 | (R)-methylmalonyl-CoA | Caldanaerobacter subterraneus subsp. tengcongensis | - |
(S)-methylmalonyl-CoA | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.99.1 | Caldanaerobacter subterraneus subsp. tengcongensis | Q8RCQ6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.99.1 | lysate is clarified by centrifugation and purified by passage through a nickel-affinity column, and a further purification step is performed by gel filtration | Caldanaerobacter subterraneus subsp. tengcongensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.99.1 | (R)-methylmalonyl-CoA | - |
Caldanaerobacter subterraneus subsp. tengcongensis | (S)-methylmalonyl-CoA | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.99.1 | dimer | in solution | Caldanaerobacter subterraneus subsp. tengcongensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.99.1 | methylmalonyl-CoA epimerase | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
| 5.1.99.1 | MMCE | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
| 5.1.99.1 | TTE0360 | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.1.99.1 | physiological function | second step in the pathway responsible for the degradation of branched amino acids and odd chain fatty acids, MMCE participated in autotrophic CO2 fixation via the 3-hydroxypropionate pathway, in propionate fermentation, the regeneration of glyoxylate and in the biosynthesis of polyketide antibiotics | Caldanaerobacter subterraneus subsp. tengcongensis |
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Release 2023.1 (January 2023)
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