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Literature summary extracted from

  • Juers, D.H.; Rob, B.; Dugdale, M.L.; Rahimzadeh, N.; Giang, C.; Lee, M.; Matthews, B.W.; Huber, R.E.
    Direct and indirect roles of His-418 in metal binding and in the activity of beta-galactosidase (E. coli) (2009), Protein Sci., 18, 1281-1292.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.23 mutant enzymes H418E and H418N Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.23 H418E the mutant shows low catalytic activity Escherichia coli
3.2.1.23 H418F the mutant shows low catalytic activity, the variant has reduced stability, binds Mg2+ weakly and cannot be crystallized Escherichia coli
3.2.1.23 H418N the mutant shows low catalytic activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 2-amino-2-deoxy-D-galactopyranose
-
 Escherichia coli
3.2.1.23 2-nitrophenyl 1-thio-beta-D-galactopyranoside
-
 Escherichia coli
3.2.1.23 2-phenylethyl 1-thio-beta-D-galactopyranoside
-
 Escherichia coli
3.2.1.23 D-galactonolactone
-
 Escherichia coli
3.2.1.23 D-galactose
-
 Escherichia coli
3.2.1.23 isopropyl-thio-beta-D-galactopyranoside
-
 Escherichia coli
3.2.1.23 L-arabinose
-
 Escherichia coli
3.2.1.23 L-ribose
-
 Escherichia coli
3.2.1.23 lactose
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.04
-
 4-nitrophenyl beta-D-galactopyranoside mutant enzyme H418N, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 0.04
-
 2-nitrophenyl beta-D-galactopyranoside wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, pH 7.0 at 25°C Escherichia coli
3.2.1.23 0.06
-
 2-nitrophenyl beta-D-galactopyranoside mutant enzyme H418N, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 0.12
-
 4-nitrophenyl beta-D-galactopyranoside wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, pH 7.0 at 25°C Escherichia coli
3.2.1.23 13.2
-
 4-nitrophenyl beta-D-galactopyranoside mutant enzyme H418E, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 20.8
-
 4-nitrophenyl beta-D-galactopyranoside mutant enzyme H418F, in 30 mM TES buffer, 10 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 30.6
-
 2-nitrophenyl beta-D-galactopyranoside mutant enzyme H418F, in 30 mM TES buffer, 10 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 104
-
 2-nitrophenyl beta-D-galactopyranoside mutant enzyme H418E, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 Mg2+ the active site of beta-galactosidase contains a Mg2+ ion ligated by Glu-416, His-418 and Glu-461 plus three water molecules Escherichia coli
3.2.1.23 Na+ the active site of beta-galactosidase contains a Na+ ion Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.23 lactose + H2O Escherichia coli
-
 D-galactose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Escherichia coli P00722
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 Superose 6 gel filtration and Superose 12 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Escherichia coli 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Escherichia coli 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Escherichia coli D-galactose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 homotetramer x-ray crystallography Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 beta-gal
-
 Escherichia coli
3.2.1.23 BGA
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 4.9
-
 2-nitrophenyl beta-D-galactopyranoside mutant enzyme H418N, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 5.4
-
 2-nitrophenyl beta-D-galactopyranoside mutant enzyme H418E, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 5.4
-
 4-nitrophenyl beta-D-galactopyranoside mutant enzyme H418E, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 5.5
-
 4-nitrophenyl beta-D-galactopyranoside mutant enzyme H418N, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 10.3
-
 4-nitrophenyl beta-D-galactopyranoside mutant enzyme H418F, in 30 mM TES buffer, 10 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 10.4
-
 2-nitrophenyl beta-D-galactopyranoside mutant enzyme H418F, in 30 mM TES buffer, 10 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 90
-
 2-nitrophenyl beta-D-galactopyranoside wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, pH 7.0 at 25°C Escherichia coli
3.2.1.23 620
-
 4-nitrophenyl beta-D-galactopyranoside wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, pH 7.0 at 25°C Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.23 0.002
-
 2-phenylethyl 1-thio-beta-D-galactopyranoside wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 0.025
-
 2-nitrophenyl 1-thio-beta-D-galactopyranoside wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 0.08
-
 2-phenylethyl 1-thio-beta-D-galactopyranoside mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 0.08
-
 isopropyl-thio-beta-D-galactopyranoside wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 0.28
-
 L-ribose wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 0.4
-
 D-galactonolactone mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 0.4
-
 D-galactonolactone mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 0.64
-
 D-galactonolactone wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 0.68
-
 L-ribose mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 0.9
-
 lactose wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 1
-
 2-amino-2-deoxy-D-galactopyranose wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 1.3
-
 2-amino-2-deoxy-D-galactopyranose mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 1.4
-
 L-ribose mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 1.4
-
 2-amino-2-deoxy-D-galactopyranose mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 2 5 L-arabinose mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 3.3
-
 D-galactose mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 3.4
-
 D-galactose mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 4.2
-
 2-nitrophenyl 1-thio-beta-D-galactopyranoside mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 5
-
 isopropyl-thio-beta-D-galactopyranoside mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 5.1
-
 lactose mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 8.4
-
 2-nitrophenyl 1-thio-beta-D-galactopyranoside mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 10
-
 2-phenylethyl 1-thio-beta-D-galactopyranoside mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 13
-
 lactose mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 21
-
 D-galactose wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 85
-
 isopropyl-thio-beta-D-galactopyranoside mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 130
-
 L-arabinose mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli
3.2.1.23 220
-
 L-arabinose wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C Escherichia coli