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Literature summary extracted from

  • Lohman, J.R.; Olson, A.C.; Remington, S.J.
    Atomic resolution structures of Escherichia coli and Bacillus anthracis malate synthase A: comparison with isoform G and implications for structure-based drug discovery (2008), Protein Sci., 17, 1935-1945.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.9 His-tag version expressed in Escherichia coli Escherichia coli
2.3.3.9 His-tagged version, gene synthesized commercially, expressed in Escherichia coli Bacillus anthracis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.3.9
-
 Escherichia coli
2.3.3.9
-
 Bacillus anthracis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.9 C438S residue in the acetyl-CoA binding pocket Escherichia coli
2.3.3.9 C617S residue in the acetyl-CoA binding pocket Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.9 2,4,5-trioxopyrrolidine-3-carboxamide competitive Escherichia coli
2.3.3.9 oxalate competitive Escherichia coli
2.3.3.9 parabanic acid competitive Escherichia coli
2.3.3.9 pyruvate
-
 Bacillus anthracis
2.3.3.9 pyruvate competitive Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.9 0.009
-
 acetyl-CoA wild-type protein Escherichia coli
2.3.3.9 0.021
-
 glyoxylate wild-type protein Escherichia coli
2.3.3.9 0.022
-
 acetyl-CoA wild-type protein Escherichia coli
2.3.3.9 0.023
-
 acetyl-CoA
-
 Bacillus anthracis
2.3.3.9 0.046
-
 acetyl-CoA C617 mutant protein Escherichia coli
2.3.3.9 0.05
-
 glyoxylate C617 mutant protein Escherichia coli
2.3.3.9 0.055
-
 glyoxylate wild-type protein Escherichia coli
2.3.3.9 0.056
-
 acetyl-CoA C438S mutant protein Escherichia coli
2.3.3.9 0.058
-
 glyoxylate C438S mutant protein Escherichia coli
2.3.3.9 0.085
-
 glyoxylate
-
 Bacillus anthracis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.9 Mg2+
-
 Escherichia coli
2.3.3.9 Mg2+
-
 Bacillus anthracis

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.9 Bacillus anthracis A0A0F7RL08
-
-
2.3.3.9 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.9
-
 Escherichia coli
2.3.3.9
-
 Bacillus anthracis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.9 additional information
-
 C438S mutant protein has 74% of wild-type acitvity Escherichia coli
2.3.3.9 additional information
-
 C617S mutant protein has 88% of wild-type acitvity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.9 acetyl-CoA + glyoxylate + H2O
-
 Escherichia coli CoA + (S)-malate
-
 ?
2.3.3.9 acetyl-CoA + glyoxylate + H2O
-
 Bacillus anthracis CoA + (S)-malate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.9 malate synthase A
-
 Escherichia coli
2.3.3.9 malate synthase A
-
 Bacillus anthracis
2.3.3.9 malate synthase G
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.9 0.023
-
 oxalate wild-type protein Escherichia coli
2.3.3.9 0.037
-
 oxalate wild-type protein Escherichia coli
2.3.3.9 0.04
-
 2,4,5-trioxopyrrolidine-3-carboxamide C438S mutant protein Escherichia coli
2.3.3.9 0.113
-
 2,4,5-trioxopyrrolidine-3-carboxamide C617 mutant protein Escherichia coli
2.3.3.9 0.37
-
 parabanic acid C438S mutant protein Escherichia coli
2.3.3.9 0.55
-
 parabanic acid C617 mutant protein Escherichia coli
2.3.3.9 0.6
-
 pyruvate C438S mutant protein Escherichia coli
2.3.3.9 0.65
-
 pyruvate wild-type protein Escherichia coli
2.3.3.9 0.75
-
 pyruvate
-
 Bacillus anthracis
2.3.3.9 1
-
 pyruvate C617 mutant protein Escherichia coli
2.3.3.9 1
-
 pyruvate wild-type protein Escherichia coli