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Literature summary extracted from

  • Wang, J.; Edmondson, D.E.
    High-level expression and purification of rat monoamine oxidase A (MAO A) in Pichia pastoris: comparison with human MAO A (2009), Protein Expr. Purif., 70, 211-217.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.4 expressed in Pichia pastoris Rattus norvegicus
1.4.3.4 overexpression of MAO-A in Pichia pastoris strain KM71 Rattus norvegicus

General Stability

EC Number General Stability Organism
1.4.3.4 50% glycerol stabilize the enzyme Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.4 Clorgyline MAO-A forms a N(5) flavocyanine adduct on inhibition by clorgyline, MAO A is blocked at an N-terminal threonyl residue Rattus norvegicus
1.4.3.4 D-amphetamine reversible inhibitor; reversible inhibitor Rattus norvegicus
1.4.3.4 isatin reversible inhibitor; reversible inhibitor Rattus norvegicus
1.4.3.4 additional information no inhibition of MAO-A by farnesol, chlorostyrylcaffeine, and 1,4-diphenyl-2-butene; not inhibited by farnesol, chlorostyrylcaffeine and 1,4-diphenyl-2-butene Rattus norvegicus
1.4.3.4 phentermine reversible inhibitor; reversible inhibitor Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.4 additional information
-
 additional information steady-state kinetic studies, recombinant enzyme, overview Rattus norvegicus
1.4.3.4 0.0093
-
 O2 isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.4.3.4 0.09
-
 kynuramine pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 0.09
-
 kynuramine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.4.3.4 0.21
-
 benzylamine pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 0.21
-
 benzylamine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.4.3.4 0.3
-
 serotonin pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 0.3
-
 serotonin isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.4.3.4 0.35
-
 Phenylethylamine pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 0.35
-
 2-Phenylethylamine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.3.4 mitochondrial membrane outer Rattus norvegicus 31966
-
1.4.3.4 mitochondrial outer membrane
-
 Rattus norvegicus 5741
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.4 60000
-
 x * 60000, recombinant MAO-A, SDS-PAGE Rattus norvegicus
1.4.3.4 60000
-
 x * 60000, isoform MAO A, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.4 benzylamine + H2O + O2 Rattus norvegicus MAO-A benzaldehyde + NH3 + H2O2
-
 ?
1.4.3.4 kynuramine + H2O + O2 Rattus norvegicus MAO-A 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2
-
 ?
1.4.3.4 phenylethylamine + H2O + O2 Rattus norvegicus MAO-A 2-phenylethanal + NH3 + H2O2
-
 ?
1.4.3.4 serotonin + H2O + O2 Rattus norvegicus MAO-A (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.4 Rattus norvegicus
-
-
-
1.4.3.4 Rattus norvegicus P21396
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.4.3.4 flavoprotein
-
 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.4 DEAE-Sepharose column chromatography Rattus norvegicus
1.4.3.4 recombinant MAO-A from Pichia pastoris strain KM71 outer mitochondrial membranes by Triton X-100 solubilization and anion exchange chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.4 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.4 0.025
-
 cell lysate, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.4.3.4 1.42
-
 purified recombinant MAO-A Rattus norvegicus
1.4.3.4 1.42
-
 after purification, isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.4 2-phenylethylamine + H2O + O2
-
 Rattus norvegicus 2-phenylethanal + NH3 + H2O2
-
 ?
1.4.3.4 benzylamine + H2O + O2
-
 Rattus norvegicus benzaldehyde + NH3 + H2O2
-
 ?
1.4.3.4 benzylamine + H2O + O2 MAO-A Rattus norvegicus benzaldehyde + NH3 + H2O2
-
 ?
1.4.3.4 kynuramine + H2O + O2
-
 Rattus norvegicus 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2
-
 ?
1.4.3.4 kynuramine + H2O + O2 MAO-A Rattus norvegicus 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2
-
 ?
1.4.3.4 phenylethylamine + H2O + O2 MAO-A Rattus norvegicus 2-phenylethanal + NH3 + H2O2
-
 ?
1.4.3.4 serotonin + H2O + O2
-
 Rattus norvegicus (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
 ?
1.4.3.4 serotonin + H2O + O2 MAO-A Rattus norvegicus (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.4 ? x * 60000, recombinant MAO-A, SDS-PAGE Rattus norvegicus
1.4.3.4 ? x * 60000, isoform MAO A, SDS-PAGE Rattus norvegicus
1.4.3.4 More peptide mapping, MALDI-TOF spectroscopic analysis, overview Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.4.3.4 MAO A
-
 Rattus norvegicus
1.4.3.4 MAO A isoform Rattus norvegicus
1.4.3.4 monoamine oxidase A
-
 Rattus norvegicus
1.4.3.4 monoamine oxidase A isoform Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.4 25
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.4
-
 30 isoform MAO A remains fully active when incubated at 0°C for 120 minutes. At 25°C, only 10% of isoform MAO A activity is lost after 120 min incubation. The enzyme retains about 80% catalytic activity after incubation at 30°C for 120 min Rattus norvegicus
1.4.3.4 25
-
 purified recombinant MAO-A, 120 min, loss of 10% activity Rattus norvegicus
1.4.3.4 30
-
 purified recombinant MAO-A, 120 min, loss of 20% activity Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.4 0.325
-
 benzylamine pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 0.325
-
 benzylamine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.4.3.4 1.42
-
 Phenylethylamine pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 1.42
-
 2-Phenylethylamine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.4.3.4 2.48
-
 kynuramine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.4.3.4 2.485
-
 kynuramine pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 5.98
-
 serotonin pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 5.98
-
 serotonin isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.4 7.5
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.4 FAD covalently bound Rattus norvegicus
1.4.3.4 FAD the enzyme contains a covalently bound FAD Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.4 0.006
-
 D-amphetamine pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 0.0105
-
 isatin pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 0.626
-
 phentermine pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 6
-
 D-amphetamine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.4.3.4 10
-
 isatin isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.4.3.4 625
-
 phentermine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.4 1.55
-
 benzylamine pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 1.55
-
 benzylamine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.4.3.4 4.05
-
 Phenylethylamine pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 4.05
-
 2-Phenylethylamine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.4.3.4 19.93
-
 serotonin pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 19.93
-
 serotonin isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.4.3.4 27.61
-
 kynuramine pH 7.5, 25°C, MAO-A Rattus norvegicus
1.4.3.4 27.61
-
 kynuramine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus