EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.2.13 | docking model of MTG binding to substrate N-carbobenzoxy-L-glutaminyl-glycine. Substrate is stretched along the MTG active site cleft with hydrophobic and/or aromatic residues interacting directly with the substrate. An oxyanion binding site for TGase activity may be constructed from the amide groups of Cys64 and/or Val65 | Streptomyces mobaraensis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.2.13 | C64A | complete loss of activity | Streptomyces mobaraensis |
2.3.2.13 | C64A | 0.4% residual activity | Streptomyces mobaraensis |
2.3.2.13 | D255A | complete loss of activity | Streptomyces mobaraensis |
2.3.2.13 | D255A | 0.2% residual activity | Streptomyces mobaraensis |
2.3.2.13 | D304A | 41.1% residual activity | Streptomyces mobaraensis |
2.3.2.13 | E300A | 54.7% residual activity | Streptomyces mobaraensis |
2.3.2.13 | F254A | complete loss of activity | Streptomyces mobaraensis |
2.3.2.13 | F305A | 18.6% residual activity | Streptomyces mobaraensis |
2.3.2.13 | G63A | complete loss of activity | Streptomyces mobaraensis |
2.3.2.13 | H274A | 9.3% residual activity | Streptomyces mobaraensis |
2.3.2.13 | H277A | complete loss of activity | Streptomyces mobaraensis |
2.3.2.13 | I240A | 68.3% residual activity | Streptomyces mobaraensis |
2.3.2.13 | L285A | 36.8% residual activity | Streptomyces mobaraensis |
2.3.2.13 | N253A | complete loss of activity | Streptomyces mobaraensis |
2.3.2.13 | N253A | 1.1% residual activity | Streptomyces mobaraensis |
2.3.2.13 | N276A | 1.7% residual activity | Streptomyces mobaraensis |
2.3.2.13 | R26A | 18% residual activity | Streptomyces mobaraensis |
2.3.2.13 | V252A | 6.0% residual activity | Streptomyces mobaraensis |
2.3.2.13 | V65A | 10.2% residual activity | Streptomyces mobaraensis |
2.3.2.13 | Y256A | complete loss of activity | Streptomyces mobaraensis |
2.3.2.13 | Y256A | 1.9% residual activity | Streptomyces mobaraensis |
2.3.2.13 | Y278A | 3.9% residual activity | Streptomyces mobaraensis |
2.3.2.13 | Y62A | complete loss of activity | Streptomyces mobaraensis |
2.3.2.13 | Y75A | 5.3% residual activity | Streptomyces mobaraensis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.2.13 | Streptomyces mobaraensis | P81453 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.13 | N-carbobenzoxy-L-glutaminyl-glycine + ovalbumin | - |
Streptomyces mobaraensis | ? | - |
? |