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Literature summary extracted from

  • Tagami, U.; Shimba, N.; Nakamura, M.; Yokoyama, K.; Suzuki, E.; Hirokawa, T.
    Substrate specificity of microbial transglutaminase as revealed by three-dimensional docking simulation and mutagenesis (2009), Protein Eng. Des. Sel., 22, 747-752.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.2.13 docking model of MTG binding to substrate N-carbobenzoxy-L-glutaminyl-glycine. Substrate is stretched along the MTG active site cleft with hydrophobic and/or aromatic residues interacting directly with the substrate. An oxyanion binding site for TGase activity may be constructed from the amide groups of Cys64 and/or Val65 Streptomyces mobaraensis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.13 C64A complete loss of activity Streptomyces mobaraensis
2.3.2.13 C64A 0.4% residual activity Streptomyces mobaraensis
2.3.2.13 D255A complete loss of activity Streptomyces mobaraensis
2.3.2.13 D255A 0.2% residual activity Streptomyces mobaraensis
2.3.2.13 D304A 41.1% residual activity Streptomyces mobaraensis
2.3.2.13 E300A 54.7% residual activity Streptomyces mobaraensis
2.3.2.13 F254A complete loss of activity Streptomyces mobaraensis
2.3.2.13 F305A 18.6% residual activity Streptomyces mobaraensis
2.3.2.13 G63A complete loss of activity Streptomyces mobaraensis
2.3.2.13 H274A 9.3% residual activity Streptomyces mobaraensis
2.3.2.13 H277A complete loss of activity Streptomyces mobaraensis
2.3.2.13 I240A 68.3% residual activity Streptomyces mobaraensis
2.3.2.13 L285A 36.8% residual activity Streptomyces mobaraensis
2.3.2.13 N253A complete loss of activity Streptomyces mobaraensis
2.3.2.13 N253A 1.1% residual activity Streptomyces mobaraensis
2.3.2.13 N276A 1.7% residual activity Streptomyces mobaraensis
2.3.2.13 R26A 18% residual activity Streptomyces mobaraensis
2.3.2.13 V252A 6.0% residual activity Streptomyces mobaraensis
2.3.2.13 V65A 10.2% residual activity Streptomyces mobaraensis
2.3.2.13 Y256A complete loss of activity Streptomyces mobaraensis
2.3.2.13 Y256A 1.9% residual activity Streptomyces mobaraensis
2.3.2.13 Y278A 3.9% residual activity Streptomyces mobaraensis
2.3.2.13 Y62A complete loss of activity Streptomyces mobaraensis
2.3.2.13 Y75A 5.3% residual activity Streptomyces mobaraensis

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Streptomyces mobaraensis P81453
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 N-carbobenzoxy-L-glutaminyl-glycine + ovalbumin
-
Streptomyces mobaraensis ?
-
?